dorsal/arxiv
View SchemaA versatile electrophoresis system for the analysis of high- and low-molecular-weight proteins
| Authors | Christophe Tastet, Pierre Lescuyer, Hélène Diemer, Sylvie Luche, Alain van Dorsselaer, Thierry Rabilloud |
|---|---|
| Categories | |
| ArXiv ID | q-bio/0611088 |
| URL | https://arxiv.org/abs/q-bio/0611088 |
| Journal | Methods in molecular biology (Clifton, N.J.) 355 (2006) 111-20 |
Abstract
A new, versatile, multiphasic buffer system for high-resolution sodium dodecyl sulfate-polyacrylamide gel electrophoresis of proteins in the relative molecular weight range of 300 000-3000 Da is described. The system, based on the theory of multiphasic zone electrophoresis, allows complete stacking and destacking of proteins in the above M(r) range. The buffer system uses taurine and chloride as trailing and leading ion, respectively, and Tris, at a pH close to its pK(a), as the buffering counterion. Coupled with limited variation in the acrylamide concentration, this electrophoresis system allows to tailor the resolution in the 6-200 kDa M(r) range, with minimal difficulties in the post electrophoretic identification processes.
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"abstract": "A new, versatile, multiphasic buffer system for high-resolution sodium\ndodecyl sulfate-polyacrylamide gel electrophoresis of proteins in the relative\nmolecular weight range of 300 000-3000 Da is described. The system, based on\nthe theory of multiphasic zone electrophoresis, allows complete stacking and\ndestacking of proteins in the above M(r) range. The buffer system uses taurine\nand chloride as trailing and leading ion, respectively, and Tris, at a pH close\nto its pK(a), as the buffering counterion. Coupled with limited variation in\nthe acrylamide concentration, this electrophoresis system allows to tailor the\nresolution in the 6-200 kDa M(r) range, with minimal difficulties in the post\nelectrophoretic identification processes.",
"arxiv_id": "q-bio/0611088",
"authors": [
"Christophe Tastet",
"Pierre Lescuyer",
"H\u00e9l\u00e8ne Diemer",
"Sylvie Luche",
"Alain van Dorsselaer",
"Thierry Rabilloud"
],
"categories": [
"q-bio.GN"
],
"journal_ref": "Methods in molecular biology (Clifton, N.J.) 355 (2006) 111-20",
"title": "A versatile electrophoresis system for the analysis of high- and low-molecular-weight proteins",
"url": "https://arxiv.org/abs/q-bio/0611088"
},
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