dorsal/arxiv
View SchemaOn the relationships between kinetic schemes and two-state single molecule trajectories
| Authors | Ophir Flomenbom, J. Klafter |
|---|---|
| Categories | |
| ArXiv ID | q-bio/0702034 |
| URL | https://arxiv.org/abs/q-bio/0702034 |
| DOI | 10.1063/1.1979489 |
| Journal | J. Chem. Phys. 123, 064903 (2005) |
Abstract
Trajectories of a signal that fluctuates between two states which originate from single molecule activities have become ubiquitous. Common examples are trajectories of ionic flux through individual membrane-channels, and of photon counts collected from diffusion, activity, and conformational changes of biopolymers. By analyzing the trajectory, one wishes to deduce the underlying mechanism, which is usually described by a multi-substate kinetic scheme. In previous works, we divided kinetic schemes that generate two-state trajectories into two types: reducible schemes and irreducible schemes. We showed that all the information in trajectories generated from reducible schemes is contained in the waiting time probability density functions (PDFs) of the two states. It follows that reducible schemes with the same waiting time PDFs are not distinguishable. In this work, we further characterize the topologies of kinetic schemes, now of irreducible schemes, and further study two-state trajectories from the two types of scheme. We suggest various methods for extracting information about the underlying kinetic scheme from the trajectory (e. g., calculate the binned successive waiting times PDF and analyze the ordered waiting times trajectory), and point out the advantages and disadvantages of each. We show that the binned successive waiting times PDF is not only more robust than other functions when analyzing finite trajectories, but contains, in most cases, more information about the underlying kinetic scheme than other functions in the limit of infinitely long trajectories. For some cases however, analyzing the ordered waiting times trajectory may supply unique information about the underlying kinetic scheme.
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"abstract": "Trajectories of a signal that fluctuates between two states which originate\nfrom single molecule activities have become ubiquitous. Common examples are\ntrajectories of ionic flux through individual membrane-channels, and of photon\ncounts collected from diffusion, activity, and conformational changes of\nbiopolymers. By analyzing the trajectory, one wishes to deduce the underlying\nmechanism, which is usually described by a multi-substate kinetic scheme. In\nprevious works, we divided kinetic schemes that generate two-state trajectories\ninto two types: reducible schemes and irreducible schemes. We showed that all\nthe information in trajectories generated from reducible schemes is contained\nin the waiting time probability density functions (PDFs) of the two states. It\nfollows that reducible schemes with the same waiting time PDFs are not\ndistinguishable. In this work, we further characterize the topologies of\nkinetic schemes, now of irreducible schemes, and further study two-state\ntrajectories from the two types of scheme. We suggest various methods for\nextracting information about the underlying kinetic scheme from the trajectory\n(e. g., calculate the binned successive waiting times PDF and analyze the\nordered waiting times trajectory), and point out the advantages and\ndisadvantages of each. We show that the binned successive waiting times PDF is\nnot only more robust than other functions when analyzing finite trajectories,\nbut contains, in most cases, more information about the underlying kinetic\nscheme than other functions in the limit of infinitely long trajectories. For\nsome cases however, analyzing the ordered waiting times trajectory may supply\nunique information about the underlying kinetic scheme.",
"arxiv_id": "q-bio/0702034",
"authors": [
"Ophir Flomenbom",
"J. Klafter"
],
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"doi": "10.1063/1.1979489",
"journal_ref": "J. Chem. Phys. 123, 064903 (2005)",
"title": "On the relationships between kinetic schemes and two-state single molecule trajectories",
"url": "https://arxiv.org/abs/q-bio/0702034"
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