dorsal/arxiv
View SchemaA versatile maskless microscope projection photolithography system and its application in light-directed fabrication of DNA microarrays
| Authors | Thomas Naiser, Timo Mai, Wolfgang Michel, Albrecht Ott |
|---|---|
| Categories | |
| ArXiv ID | q-bio/0608038 |
| URL | https://arxiv.org/abs/q-bio/0608038 |
| DOI | 10.1063/1.2213152 |
| Journal | Rev. Sci. Instrum. 77, 063711 (2006) |
Abstract
We present a maskless microscope projection lithography system (MPLS), in which photomasks have been replaced by a Digital Micromirror Device type spatial light modulator (DMD, Texas Instruments). Employing video projector technology high resolution patterns, designed as bitmap images on the computer, are displayed using a micromirror array consisting of about 786000 tiny individually addressable tilting mirrors. The DMD, which is located in the image plane of an infinity corrected microscope, is projected onto a substrate placed in the focal plane of the microscope objective. With a 5x(0.25 NA) Fluar microscope objective, a fivefold reduction of the image to a total size of 9 mm2 and a minimum feature size of 3.5 microns is achieved. Our system can be used in the visible range as well as in the near UV (with a light intensity of up to 76 mW/cm2 around the 365 nm Hg-line). We developed an inexpensive and simple method to enable exact focusing and controlling of the image quality of the projected patterns. Our MPLS has originally been designed for the light-directed in situ synthesis of DNA microarrays. One requirement is a high UV intensity to keep the fabrication process reasonably short. Another demand is a sufficient contrast ratio over small distances (of about 5 microns). This is necessary to achieve a high density of features (i.e. separated sites on the substrate at which different DNA sequences are synthesized in parallel fashion) while at the same time the number of stray light induced DNA sequence errors is kept reasonably small. We demonstrate the performance of the apparatus in light-directed DNA chip synthesis and discuss its advantages and limitations.
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"abstract": "We present a maskless microscope projection lithography system (MPLS), in\nwhich photomasks have been replaced by a Digital Micromirror Device type\nspatial light modulator (DMD, Texas Instruments). Employing video projector\ntechnology high resolution patterns, designed as bitmap images on the computer,\nare displayed using a micromirror array consisting of about 786000 tiny\nindividually addressable tilting mirrors. The DMD, which is located in the\nimage plane of an infinity corrected microscope, is projected onto a substrate\nplaced in the focal plane of the microscope objective. With a 5x(0.25 NA) Fluar\nmicroscope objective, a fivefold reduction of the image to a total size of 9\nmm2 and a minimum feature size of 3.5 microns is achieved. Our system can be\nused in the visible range as well as in the near UV (with a light intensity of\nup to 76 mW/cm2 around the 365 nm Hg-line). We developed an inexpensive and\nsimple method to enable exact focusing and controlling of the image quality of\nthe projected patterns. Our MPLS has originally been designed for the\nlight-directed in situ synthesis of DNA microarrays. One requirement is a high\nUV intensity to keep the fabrication process reasonably short. Another demand\nis a sufficient contrast ratio over small distances (of about 5 microns). This\nis necessary to achieve a high density of features (i.e. separated sites on the\nsubstrate at which different DNA sequences are synthesized in parallel fashion)\nwhile at the same time the number of stray light induced DNA sequence errors is\nkept reasonably small. We demonstrate the performance of the apparatus in\nlight-directed DNA chip synthesis and discuss its advantages and limitations.",
"arxiv_id": "q-bio/0608038",
"authors": [
"Thomas Naiser",
"Timo Mai",
"Wolfgang Michel",
"Albrecht Ott"
],
"categories": [
"q-bio.QM",
"q-bio.BM"
],
"doi": "10.1063/1.2213152",
"journal_ref": "Rev. Sci. Instrum. 77, 063711 (2006)",
"title": "A versatile maskless microscope projection photolithography system and its application in light-directed fabrication of DNA microarrays",
"url": "https://arxiv.org/abs/q-bio/0608038"
},
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