dorsal/arxiv
View SchemaSystematic identification of abundant A-to-I editing sites in the human transcriptome
| Authors | Erez Y. Levanon, Eli Eisenberg, Rodrigo Yelin, Sergey Nemzer, Martina Hallegger, Ronen Shemesh, Zipora Y. Fligelman, Avi Shoshan, Sarah R. Pollock, Dan Sztybel, Moshe Olshansky, Gideon Rechavi, Michael F. Jantsch |
|---|---|
| Categories | |
| ArXiv ID | q-bio/0411045 |
| URL | https://arxiv.org/abs/q-bio/0411045 |
| DOI | 10.1038/nbt996 |
| Journal | Nature Biotechnology 22, 1001-1005 (2004) |
Abstract
RNA editing by members of the double-stranded RNA-specific ADAR family leads to site-specific conversion of adenosine to inosine (A-to-I) in precursor messenger RNAs. Editing by ADARs is believed to occur in all metazoa, and is essential for mammalian development. Currently, only a limited number of human ADAR substrates are known, while indirect evidence suggests a substantial fraction of all pre-mRNAs being affected. Here we describe a computational search for ADAR editing sites in the human transcriptome, using millions of available expressed sequences. 12,723 A-to-I editing sites were mapped in 1,637 different genes, with an estimated accuracy of 95%, raising the number of known editing sites by two orders of magnitude. We experimentally validated our method by verifying the occurrence of editing in 26 novel substrates. A-to-I editing in humans primarily occurs in non-coding regions of the RNA, typically in Alu repeats. Analysis of the large set of editing sites indicates the role of editing in controlling dsRNA stability.
{
"annotation_id": "f1741fdb-e8b9-4906-b8c6-0f37ee32536c",
"date_created": "2026-03-02T18:01:32.336000Z",
"date_modified": "2026-03-02T18:01:32.336000Z",
"file_hash": "cbe5fda18710c88ab50ab4840c2241647f7850dd183b6c30444792129b25fb0c",
"private": false,
"record": {
"abstract": "RNA editing by members of the double-stranded RNA-specific ADAR family leads\nto site-specific conversion of adenosine to inosine (A-to-I) in precursor\nmessenger RNAs. Editing by ADARs is believed to occur in all metazoa, and is\nessential for mammalian development. Currently, only a limited number of human\nADAR substrates are known, while indirect evidence suggests a substantial\nfraction of all pre-mRNAs being affected. Here we describe a computational\nsearch for ADAR editing sites in the human transcriptome, using millions of\navailable expressed sequences. 12,723 A-to-I editing sites were mapped in 1,637\ndifferent genes, with an estimated accuracy of 95%, raising the number of known\nediting sites by two orders of magnitude. We experimentally validated our\nmethod by verifying the occurrence of editing in 26 novel substrates. A-to-I\nediting in humans primarily occurs in non-coding regions of the RNA, typically\nin Alu repeats. Analysis of the large set of editing sites indicates the role\nof editing in controlling dsRNA stability.",
"arxiv_id": "q-bio/0411045",
"authors": [
"Erez Y. Levanon",
"Eli Eisenberg",
"Rodrigo Yelin",
"Sergey Nemzer",
"Martina Hallegger",
"Ronen Shemesh",
"Zipora Y. Fligelman",
"Avi Shoshan",
"Sarah R. Pollock",
"Dan Sztybel",
"Moshe Olshansky",
"Gideon Rechavi",
"Michael F. Jantsch"
],
"categories": [
"q-bio.GN"
],
"doi": "10.1038/nbt996",
"journal_ref": "Nature Biotechnology 22, 1001-1005 (2004)",
"title": "Systematic identification of abundant A-to-I editing sites in the human transcriptome",
"url": "https://arxiv.org/abs/q-bio/0411045"
},
"schema_id": "dorsal/arxiv",
"source": {
"execution_id": "9d051fb5-dae1-4422-ab96-452c412b2f55",
"id": "arXiv Dataset IDs",
"type": "Model",
"variant": "snapshot-2026-03-01",
"version": "0.1.0"
},
"user_id": 1000002
}