dorsal/arxiv
View SchemaElectrophoresis of positioned nucleosomes
| Authors | Martin Castelnovo, Sébastian Grauwin |
|---|---|
| Categories | |
| ArXiv ID | physics/0701036 |
| URL | https://arxiv.org/abs/physics/0701036 |
| DOI | 10.1529/biophysj.106.101014 |
Abstract
We present in this paper an original approach to compute the electrophoretic mobility of rigid nucleo-protein complexes like nucleosomes. This model allows to address theoretically the influence of complex position along DNA, as well as wrapped length of DNA on the electrophoretic mobility of the complex. The predictions of the model are in qualitative agreement with experimental results on mononucleosomes assembled on short DNA fragments (<400bp). Influence of additional experimental parameters like gel concentration, ionic strength, effective charges is also discussed in the framework of the model, and is found to be qualitatively consistent with experiments when available. Based on the present model, we propose a simple semi-empirical formula describing positioning of nucleosomes as seen through electrophoresis.
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"abstract": "We present in this paper an original approach to compute the electrophoretic\nmobility of rigid nucleo-protein complexes like nucleosomes. This model allows\nto address theoretically the influence of complex position along DNA, as well\nas wrapped length of DNA on the electrophoretic mobility of the complex. The\npredictions of the model are in qualitative agreement with experimental results\non mononucleosomes assembled on short DNA fragments (\u003c400bp). Influence of\nadditional experimental parameters like gel concentration, ionic strength,\neffective charges is also discussed in the framework of the model, and is found\nto be qualitatively consistent with experiments when available. Based on the\npresent model, we propose a simple semi-empirical formula describing\npositioning of nucleosomes as seen through electrophoresis.",
"arxiv_id": "physics/0701036",
"authors": [
"Martin Castelnovo",
"S\u00e9bastian Grauwin"
],
"categories": [
"physics.bio-ph"
],
"doi": "10.1529/biophysj.106.101014",
"title": "Electrophoresis of positioned nucleosomes",
"url": "https://arxiv.org/abs/physics/0701036"
},
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