dorsal/arxiv
View SchemaQuantitative photoluminescence of broad band absorbing melanins: A procedure to correct for inner filter and re-absorption effects
| Authors | Jennifer Riesz, Joel Gilmore, Paul Meredith |
|---|---|
| Categories | |
| ArXiv ID | physics/0408041 |
| URL | https://arxiv.org/abs/physics/0408041 |
| DOI | 10.1016/j.saa.2004.08.014 |
Abstract
We report methods for correcting the photoluminescence emission and excitation spectra of highly absorbing samples for re-absorption and inner filter effects. We derive the general form of the correction, and investigate various methods for determining the parameters. Additionally, the correction methods are tested with highly absorbing fluorescein and melanin (broadband absorption) solutions; the expected linear relationships between absorption and emission are recovered upon application of the correction, indicating that the methods are valid. These procedures allow accurate quantitative analysis of the emission of low quantum yield samples (such as melanin) at concentrations where absorption is significant.
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"abstract": "We report methods for correcting the photoluminescence emission and\nexcitation spectra of highly absorbing samples for re-absorption and inner\nfilter effects. We derive the general form of the correction, and investigate\nvarious methods for determining the parameters. Additionally, the correction\nmethods are tested with highly absorbing fluorescein and melanin (broadband\nabsorption) solutions; the expected linear relationships between absorption and\nemission are recovered upon application of the correction, indicating that the\nmethods are valid. These procedures allow accurate quantitative analysis of the\nemission of low quantum yield samples (such as melanin) at concentrations where\nabsorption is significant.",
"arxiv_id": "physics/0408041",
"authors": [
"Jennifer Riesz",
"Joel Gilmore",
"Paul Meredith"
],
"categories": [
"physics.bio-ph"
],
"doi": "10.1016/j.saa.2004.08.014",
"title": "Quantitative photoluminescence of broad band absorbing melanins: A procedure to correct for inner filter and re-absorption effects",
"url": "https://arxiv.org/abs/physics/0408041"
},
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