dorsal/arxiv
View SchemaUtilizing of protein splicing phenomenon for optimization of obtaining and purification of the recombinant human growth hormone
| Authors | P. L. Starokadomskyy, O. V. Okunev, L. V. Dubey |
|---|---|
| Categories | |
| ArXiv ID | q-bio/0612007 |
| URL | https://arxiv.org/abs/q-bio/0612007 |
| Journal | Europ J Medic Res., 2006, Oct. 8, vol.11 - p. 128-129 |
Abstract
Protein splicing is a post-translational autocatalystic excision of internal protein sequence (intein) with the subsequent ligation of the flanking polypeptides (exteins). The high specificity of excision ensured by intein makes it possible to use a phenomenon of protein splicing for the biotechnology purposes. The aim of this work was optimization of obtaining and purification of the recombinant human growth hormone using the protein splicing. It was experimentally demonstrated that the use of modified intein as auto-removal affine marker makes it possible to perform the rapid and cheap isolation of the recombinant protein Hgh. Furthermore, this approach allows to obtain the human growth hormone with native N-terminus, without formyl-metionine. Key words: intein, human growth hormone, protein splicing
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"abstract": "Protein splicing is a post-translational autocatalystic excision of internal\nprotein sequence (intein) with the subsequent ligation of the flanking\npolypeptides (exteins). The high specificity of excision ensured by intein\nmakes it possible to use a phenomenon of protein splicing for the biotechnology\npurposes. The aim of this work was optimization of obtaining and purification\nof the recombinant human growth hormone using the protein splicing. It was\nexperimentally demonstrated that the use of modified intein as auto-removal\naffine marker makes it possible to perform the rapid and cheap isolation of the\nrecombinant protein Hgh. Furthermore, this approach allows to obtain the human\ngrowth hormone with native N-terminus, without formyl-metionine. Key words:\nintein, human growth hormone, protein splicing",
"arxiv_id": "q-bio/0612007",
"authors": [
"P. L. Starokadomskyy",
"O. V. Okunev",
"L. V. Dubey"
],
"categories": [
"q-bio.BM",
"q-bio.SC"
],
"journal_ref": "Europ J Medic Res., 2006, Oct. 8, vol.11 - p. 128-129",
"title": "Utilizing of protein splicing phenomenon for optimization of obtaining and purification of the recombinant human growth hormone",
"url": "https://arxiv.org/abs/q-bio/0612007"
},
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