dorsal/arxiv
View SchemaAuto-reverse nuclear migration in bipolar mammalian cells on micropatterned surfaces
| Authors | B. Szabo, Zs. Kornyei, J. Zach, D. Selmeczi, G. Csucs, A. Czirok, T. Vicsek |
|---|---|
| Categories | |
| ArXiv ID | q-bio/0310014 |
| URL | https://arxiv.org/abs/q-bio/0310014 |
| Journal | Cell Motility and the Cytoskeleton 59(1), 38-49 (2004) |
Abstract
A novel assay based on micropatterning and time-lapse microscopy has been developed for the study of nuclear migration dynamics in cultured mammalian cells. When cultured on 10-20 um wide adhesive stripes, the motility of C6 glioma and primary mouse fibroblast cells is diminished. Nevertheless, nuclei perform an unexpected auto-reverse motion: when a migrating nucleus approaches the leading edge, it decelerates, changes the direction of motion and accelerates to move toward the other end of the elongated cell. During this process cells show signs of polarization closely following the direction of nuclear movement. The observed nuclear movement requires a functioning microtubular system, as revealed by experiments disrupting the main cytoskeletal components with specific drugs. On the basis of our results we argue that auto-reverse nuclear migration is due to forces determined by the interplay of microtubule dynamics and the changing position of the microtubule organizing center as the nucleus reaches the leading edge. Our assay recapitulates specific features of nuclear migration (cell polarization, oscillatory nuclear movement), while allows the systematic study of a large number of individual cells. In particular, our experiments yielded the first direct evidence of reversive nuclear motion in mammalian cells, induced by attachment constraints.
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"abstract": "A novel assay based on micropatterning and time-lapse microscopy has been\ndeveloped for the study of nuclear migration dynamics in cultured mammalian\ncells. When cultured on 10-20 um wide adhesive stripes, the motility of C6\nglioma and primary mouse fibroblast cells is diminished. Nevertheless, nuclei\nperform an unexpected auto-reverse motion: when a migrating nucleus approaches\nthe leading edge, it decelerates, changes the direction of motion and\naccelerates to move toward the other end of the elongated cell. During this\nprocess cells show signs of polarization closely following the direction of\nnuclear movement. The observed nuclear movement requires a functioning\nmicrotubular system, as revealed by experiments disrupting the main\ncytoskeletal components with specific drugs. On the basis of our results we\nargue that auto-reverse nuclear migration is due to forces determined by the\ninterplay of microtubule dynamics and the changing position of the microtubule\norganizing center as the nucleus reaches the leading edge. Our assay\nrecapitulates specific features of nuclear migration (cell polarization,\noscillatory nuclear movement), while allows the systematic study of a large\nnumber of individual cells. In particular, our experiments yielded the first\ndirect evidence of reversive nuclear motion in mammalian cells, induced by\nattachment constraints.",
"arxiv_id": "q-bio/0310014",
"authors": [
"B. Szabo",
"Zs. Kornyei",
"J. Zach",
"D. Selmeczi",
"G. Csucs",
"A. Czirok",
"T. Vicsek"
],
"categories": [
"q-bio.CB"
],
"journal_ref": "Cell Motility and the Cytoskeleton 59(1), 38-49 (2004)",
"title": "Auto-reverse nuclear migration in bipolar mammalian cells on micropatterned surfaces",
"url": "https://arxiv.org/abs/q-bio/0310014"
},
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