dorsal/arxiv
View SchemaEvaluation of nonionic and zwitterionic detergents as membrane protein solubilizers in two-dimensional electrophoresis
| Authors | Sylvie Luche, Véronique Santoni, Thierry Rabilloud |
|---|---|
| Categories | |
| ArXiv ID | q-bio/0611076 |
| URL | https://arxiv.org/abs/q-bio/0611076 |
| DOI | 10.1002/pmic.200390037 |
| Journal | Proteomics 3 (03/2003) 249-53 |
Abstract
The solubilizing power of various nonionic and zwitterionic detergents as membrane protein solubilizers for two-dimensional electrophoresis was investigated. Human red blood cell ghosts and Arabidopsis thaliana leaf membrane proteins were used as model systems. Efficient detergents could be found in each class, i.e. with oligooxyethylene, sugar or sulfobetaine polar heads. Among the commercially available nonionic detergents, dodecyl maltoside and decaethylene glycol mono hexadecyl ether proved most efficient. They complement the more classical sulfobetaine detergents to widen the scope of useful detergents for the solubilization of membrane proteins in proteomics.
{
"annotation_id": "dfa7256e-1a5c-45de-a788-744c7a076ff4",
"date_created": "2026-03-02T18:01:34.731000Z",
"date_modified": "2026-03-02T18:01:34.731000Z",
"file_hash": "6030b07acecdd4979b09fe5910f4d0e1de208feb42e30f76ac91ecb72b963328",
"private": false,
"record": {
"abstract": "The solubilizing power of various nonionic and zwitterionic detergents as\nmembrane protein solubilizers for two-dimensional electrophoresis was\ninvestigated. Human red blood cell ghosts and Arabidopsis thaliana leaf\nmembrane proteins were used as model systems. Efficient detergents could be\nfound in each class, i.e. with oligooxyethylene, sugar or sulfobetaine polar\nheads. Among the commercially available nonionic detergents, dodecyl maltoside\nand decaethylene glycol mono hexadecyl ether proved most efficient. They\ncomplement the more classical sulfobetaine detergents to widen the scope of\nuseful detergents for the solubilization of membrane proteins in proteomics.",
"arxiv_id": "q-bio/0611076",
"authors": [
"Sylvie Luche",
"V\u00e9ronique Santoni",
"Thierry Rabilloud"
],
"categories": [
"q-bio.GN"
],
"doi": "10.1002/pmic.200390037",
"journal_ref": "Proteomics 3 (03/2003) 249-53",
"title": "Evaluation of nonionic and zwitterionic detergents as membrane protein solubilizers in two-dimensional electrophoresis",
"url": "https://arxiv.org/abs/q-bio/0611076"
},
"schema_id": "dorsal/arxiv",
"source": {
"execution_id": "04a91934-e2c3-41ca-bb26-bb717a7eb2e9",
"id": "arXiv Dataset IDs",
"type": "Model",
"variant": "snapshot-2026-03-01",
"version": "0.1.0"
},
"user_id": 1000002
}