dorsal/arxiv
View SchemaHigh expression of antioxidant proteins in dendritic cells: possible implications in atherosclerosis
| Authors | Aymeric Rivollier, Laure Perrin-Cocon, Sylvie Luche, Hélène Diemer, Jean-Marc Strub, Daniel Hanau, Alain van Dorsselaer, Vincent Lotteau, Chantal Rabourdin-Combe, Thierry Rabilloud, Christine Servet-Delprat |
|---|---|
| Categories | |
| ArXiv ID | q-bio/0611082 |
| URL | https://arxiv.org/abs/q-bio/0611082 |
| DOI | 10.1074/mcp.M500262-MCP200 |
| Journal | Mol Cell Proteomics 5 (04/2006) 726-36 |
Abstract
Dendritic cells (DCs) display the unique ability to activate naive T cells and to initiate primary T cell responses revealed in DC-T cell alloreactions. DCs frequently operate under stress conditions. Oxidative stress enhances the production of inflammatory cytokines by DCs. We performed a proteomic analysis to see which major changes occur, at the protein expression level, during DC differentiation and maturation. Comparative two-dimensional gel analysis of the monocyte, immature DC, and mature DC stages was performed. Manganese superoxide dismutase (Mn-SOD) reached 0.7% of the gel-displayed proteins at the mature DC stage. This important amount of Mn-SOD is a primary antioxidant defense system against superoxide radicals, but its product, H(2)O(2), is also deleterious for cells. Peroxiredoxin (Prx) enzymes play an important role in eliminating such peroxide. Prx1 expression level continuously increased during DC differentiation and maturation, whereas Prx6 continuously decreased, and Prx2 peaked at the immature DC stage. As a consequence, DCs were more resistant than monocytes to apoptosis induced by high amounts of oxidized low density lipoproteins containing toxic organic peroxides and hydrogen peroxide. Furthermore DC-stimulated T cells produced high levels of receptor activator of nuclear factor kappaB ligand, a chemotactic and survival factor for monocytes and DCs. This study provides insights into the original ability of DCs to express very high levels of antioxidant enzymes such as Mn-SOD and Prx1, to detoxify oxidized low density lipoproteins, and to induce high levels of receptor activator of nuclear factor kappaB ligand by the T cells they activate and further emphasizes the role that DCs might play in atherosclerosis, a pathology recognized as a chronic inflammatory disorder.
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"abstract": "Dendritic cells (DCs) display the unique ability to activate naive T cells\nand to initiate primary T cell responses revealed in DC-T cell alloreactions.\nDCs frequently operate under stress conditions. Oxidative stress enhances the\nproduction of inflammatory cytokines by DCs. We performed a proteomic analysis\nto see which major changes occur, at the protein expression level, during DC\ndifferentiation and maturation. Comparative two-dimensional gel analysis of the\nmonocyte, immature DC, and mature DC stages was performed. Manganese superoxide\ndismutase (Mn-SOD) reached 0.7% of the gel-displayed proteins at the mature DC\nstage. This important amount of Mn-SOD is a primary antioxidant defense system\nagainst superoxide radicals, but its product, H(2)O(2), is also deleterious for\ncells. Peroxiredoxin (Prx) enzymes play an important role in eliminating such\nperoxide. Prx1 expression level continuously increased during DC\ndifferentiation and maturation, whereas Prx6 continuously decreased, and Prx2\npeaked at the immature DC stage. As a consequence, DCs were more resistant than\nmonocytes to apoptosis induced by high amounts of oxidized low density\nlipoproteins containing toxic organic peroxides and hydrogen peroxide.\nFurthermore DC-stimulated T cells produced high levels of receptor activator of\nnuclear factor kappaB ligand, a chemotactic and survival factor for monocytes\nand DCs. This study provides insights into the original ability of DCs to\nexpress very high levels of antioxidant enzymes such as Mn-SOD and Prx1, to\ndetoxify oxidized low density lipoproteins, and to induce high levels of\nreceptor activator of nuclear factor kappaB ligand by the T cells they activate\nand further emphasizes the role that DCs might play in atherosclerosis, a\npathology recognized as a chronic inflammatory disorder.",
"arxiv_id": "q-bio/0611082",
"authors": [
"Aymeric Rivollier",
"Laure Perrin-Cocon",
"Sylvie Luche",
"H\u00e9l\u00e8ne Diemer",
"Jean-Marc Strub",
"Daniel Hanau",
"Alain van Dorsselaer",
"Vincent Lotteau",
"Chantal Rabourdin-Combe",
"Thierry Rabilloud",
"Christine Servet-Delprat"
],
"categories": [
"q-bio.GN"
],
"doi": "10.1074/mcp.M500262-MCP200",
"journal_ref": "Mol Cell Proteomics 5 (04/2006) 726-36",
"title": "High expression of antioxidant proteins in dendritic cells: possible implications in atherosclerosis",
"url": "https://arxiv.org/abs/q-bio/0611082"
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