dorsal/arxiv
View SchemaQuantification and Correction of Systematic Errors Due to Detector Time-Averaging in Single-Molecule Tracking Experiments
| Authors | Nicolas Destainville, Laurence Salome |
|---|---|
| Categories | |
| ArXiv ID | q-bio/0601010 |
| URL | https://arxiv.org/abs/q-bio/0601010 |
| DOI | 10.1529/biophysj.105.075176 |
| Journal | Biophys. J. 90, L17-L19 (2006) |
Abstract
Single-molecule tracking is a powerful way to look at the dynamic organization of plasma membranes. However, there are some limitations to its use. For example, it was recently observed, using numerical simulation, that time-averaging effects inherent to the exposure time of detectors are likely to bias the apparent motion of molecules confined in microdomains. Here, we solve this apparently limiting issue analytically. We explore this phenomenon by calculating its effects on the observed diffusion coefficients and domain sizes. We demonstrate that the real parameters can be easily recovered from the measured apparent ones. Interestingly, we find that single-molecule tracking can be used to explore events occurring at a timescale smaller than the exposure time.
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"abstract": "Single-molecule tracking is a powerful way to look at the dynamic\norganization of plasma membranes. However, there are some limitations to its\nuse. For example, it was recently observed, using numerical simulation, that\ntime-averaging effects inherent to the exposure time of detectors are likely to\nbias the apparent motion of molecules confined in microdomains. Here, we solve\nthis apparently limiting issue analytically. We explore this phenomenon by\ncalculating its effects on the observed diffusion coefficients and domain\nsizes. We demonstrate that the real parameters can be easily recovered from the\nmeasured apparent ones. Interestingly, we find that single-molecule tracking\ncan be used to explore events occurring at a timescale smaller than the\nexposure time.",
"arxiv_id": "q-bio/0601010",
"authors": [
"Nicolas Destainville",
"Laurence Salome"
],
"categories": [
"q-bio.QM",
"cond-mat.stat-mech",
"q-bio.SC"
],
"doi": "10.1529/biophysj.105.075176",
"journal_ref": "Biophys. J. 90, L17-L19 (2006)",
"title": "Quantification and Correction of Systematic Errors Due to Detector Time-Averaging in Single-Molecule Tracking Experiments",
"url": "https://arxiv.org/abs/q-bio/0601010"
},
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