dorsal/arxiv
View SchemaIce Formation in Model Biological Membranes in the Presence of Cryoprotectors
| Authors | M. A. Kiselev, P. Lesieur, A. M. Kisselev, M. Ollivon |
|---|---|
| Categories | |
| ArXiv ID | physics/0108023 |
| URL | https://arxiv.org/abs/physics/0108023 |
| DOI | 10.1016/S0168-9002(99)00730-5 |
| Journal | Nuclear Instruments & Methods in Physics Research A 448 (2000) 225-260 |
Abstract
Ice formation in model biological membranes is studied by SAXS and WAXS in the presence of cryoprotectors: dimethyl sulfoxide and glycerol. Three types of phospholipid membranes: DPPC, DMPC, DSPC are chosen for the investigation as well-studied model biological membranes. A special cryostat is used for sample cooling from 14.1C to -55.4C. The ice formation is only detected by WAXS in binary phospholipid/water and ternary phospholipid/cryoprotector/water systems in the condition of excess solvent. Ice formation in a binary phospholipid/water system creates an abrupt decrease of the membrane repeat distance by delta-d, so-called ice-induced dehydration of intermembrane space. The value of delta-d decreases as the cryoprotector concentration increases. The formation of ice does not influence the membrane structure (delta-d = 0) for cryoprotector mole fractions higher than 0.05.
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"abstract": "Ice formation in model biological membranes is studied by SAXS and WAXS in\nthe presence of cryoprotectors: dimethyl sulfoxide and glycerol. Three types of\nphospholipid membranes: DPPC, DMPC, DSPC are chosen for the investigation as\nwell-studied model biological membranes. A special cryostat is used for sample\ncooling from 14.1C to -55.4C. The ice formation is only detected by WAXS in\nbinary phospholipid/water and ternary phospholipid/cryoprotector/water systems\nin the condition of excess solvent. Ice formation in a binary\nphospholipid/water system creates an abrupt decrease of the membrane repeat\ndistance by delta-d, so-called ice-induced dehydration of intermembrane space.\nThe value of delta-d decreases as the cryoprotector concentration increases.\nThe formation of ice does not influence the membrane structure (delta-d = 0)\nfor cryoprotector mole fractions higher than 0.05.",
"arxiv_id": "physics/0108023",
"authors": [
"M. A. Kiselev",
"P. Lesieur",
"A. M. Kisselev",
"M. Ollivon"
],
"categories": [
"physics.bio-ph",
"cond-mat.soft",
"physics.chem-ph",
"physics.med-ph"
],
"doi": "10.1016/S0168-9002(99)00730-5",
"journal_ref": "Nuclear Instruments \u0026 Methods in Physics Research A 448 (2000)\n 225-260",
"title": "Ice Formation in Model Biological Membranes in the Presence of Cryoprotectors",
"url": "https://arxiv.org/abs/physics/0108023"
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