dorsal/arxiv
View SchemaMonitoring of the Membrane Potential in Proteoliposomes with Incorporated Cytochrome-c Oxidase Using the Fluorescent Dye Indocyanine
| Authors | Yu. A. Ivashchuk-Kienbaum |
|---|---|
| Categories | |
| ArXiv ID | physics/0211011 |
| URL | https://arxiv.org/abs/physics/0211011 |
| Journal | Ivashchuk-Kienbaum Yu. A. J. Membrane Biol. 151, 247-259, 1996 |
Abstract
A method has been developed to monitor changes of the membrane potential across vesicle membranes in real time. Using the potential-sensitive fluorescent dye indocyanine and on the basis of water/lipid redistribution model, a calculation procedure has been introduced to estimate the membrane potential in vesicles with incorporated cytochrome-c oxidase. Physical parameters, such as vesicle size distribution and density of the lipid bilayer were estimated and used as calculation parameters. By extrapolation of transient potential change to zero time, the initial rate of the potential change (dU/dt) has been calculated. It is also shown, that the initial potential change (dU/dt) may be used to study the proton/electron stoichiometry of cytochrome-c oxidase incorporated in the vesicles.
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"abstract": "A method has been developed to monitor changes of the membrane potential\nacross vesicle membranes in real time. Using the potential-sensitive\nfluorescent dye indocyanine and on the basis of water/lipid redistribution\nmodel, a calculation procedure has been introduced to estimate the membrane\npotential in vesicles with incorporated cytochrome-c oxidase. Physical\nparameters, such as vesicle size distribution and density of the lipid bilayer\nwere estimated and used as calculation parameters. By extrapolation of\ntransient potential change to zero time, the initial rate of the potential\nchange (dU/dt) has been calculated. It is also shown, that the initial\npotential change (dU/dt) may be used to study the proton/electron stoichiometry\nof cytochrome-c oxidase incorporated in the vesicles.",
"arxiv_id": "physics/0211011",
"authors": [
"Yu. A. Ivashchuk-Kienbaum"
],
"categories": [
"physics.bio-ph",
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],
"journal_ref": "Ivashchuk-Kienbaum Yu. A. J. Membrane Biol. 151, 247-259, 1996",
"title": "Monitoring of the Membrane Potential in Proteoliposomes with Incorporated Cytochrome-c Oxidase Using the Fluorescent Dye Indocyanine",
"url": "https://arxiv.org/abs/physics/0211011"
},
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