dorsal/arxiv
View SchemaArrangement of a 4Pi microscope for reducing the confocal detection volume with two-photon excitation
| Authors | N. Sandeau, H. Giovannini |
|---|---|
| Categories | |
| ArXiv ID | physics/0610036 |
| URL | https://arxiv.org/abs/physics/0610036 |
| DOI | 10.1016/j.optcom.2006.02.017 |
| Journal | Optics Communications 264 (2006) 123-129 |
Abstract
The main advantage of two-photon fluorescence confocal microscopy is the low absorption obtained with live tissues at the wavelengths of operation. However, the resolution of two-photon fluorescence confocal microscopes is lower than in the case of one-photon excitation. The 4Pi microscope type C working in two-photon regime, in which the excitation beams are coherently superimposed and, simultaneously, the emitted beams are also coherently added, has shown to be a good solution for increasing the resolution along the optical axis and for reducing the amplitude of the side lobes of the point spread function. However, the resolution in the transverse plane is poorer than in the case of one-photon excitation due to the larger wavelength involved in the two-photon fluorescence process. In this paper we show that a particular arrangement of the 4Pi microscope, referenced as 4Pi0 microscope, is a solution for obtaining a lateral resolution in the two-photon regime similar or even better to that obtained with 4Pi microscopes working in the one-photon excitation regime.
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"abstract": "The main advantage of two-photon fluorescence confocal microscopy is the low\nabsorption obtained with live tissues at the wavelengths of operation. However,\nthe resolution of two-photon fluorescence confocal microscopes is lower than in\nthe case of one-photon excitation. The 4Pi microscope type C working in\ntwo-photon regime, in which the excitation beams are coherently superimposed\nand, simultaneously, the emitted beams are also coherently added, has shown to\nbe a good solution for increasing the resolution along the optical axis and for\nreducing the amplitude of the side lobes of the point spread function. However,\nthe resolution in the transverse plane is poorer than in the case of one-photon\nexcitation due to the larger wavelength involved in the two-photon fluorescence\nprocess. In this paper we show that a particular arrangement of the 4Pi\nmicroscope, referenced as 4Pi0 microscope, is a solution for obtaining a\nlateral resolution in the two-photon regime similar or even better to that\nobtained with 4Pi microscopes working in the one-photon excitation regime.",
"arxiv_id": "physics/0610036",
"authors": [
"N. Sandeau",
"H. Giovannini"
],
"categories": [
"physics.bio-ph",
"physics.comp-ph"
],
"doi": "10.1016/j.optcom.2006.02.017",
"journal_ref": "Optics Communications 264 (2006) 123-129",
"title": "Arrangement of a 4Pi microscope for reducing the confocal detection volume with two-photon excitation",
"url": "https://arxiv.org/abs/physics/0610036"
},
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