dorsal/arxiv
View SchemaDetergents and Chaotropes for Protein Solubilization before Two-Dimensional Electrophoresis
| Authors | Thierry Rabilloud, Sylvie Luche, Véronique Santoni, Mireille Chevallet |
|---|---|
| Categories | |
| ArXiv ID | q-bio/0611077 |
| URL | https://arxiv.org/abs/q-bio/0611077 |
| Journal | Methods Mol Biol 355 (2006) 111-20 |
Abstract
Because of the outstanding separating capabilities of two-dimensional electrophoresis for complete proteins, it would be advantageous to be able to apply it to all types of proteins. Unfortunately, severe solubility problems hamper the analysis of many classes of proteins, but especially membrane proteins. These problems arise mainly in the extraction and isoelectric focusing steps, and solutions are sought to improve protein solubility under the conditions prevailing during isoelectric focusing. These solutions deal mainly with chaotropes and new detergents, which are both able to enhance protein solubility. The input of these compounds in proteomics analysis of membrane proteins is discussed, as well as future directions.
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"abstract": "Because of the outstanding separating capabilities of two-dimensional\nelectrophoresis for complete proteins, it would be advantageous to be able to\napply it to all types of proteins. Unfortunately, severe solubility problems\nhamper the analysis of many classes of proteins, but especially membrane\nproteins. These problems arise mainly in the extraction and isoelectric\nfocusing steps, and solutions are sought to improve protein solubility under\nthe conditions prevailing during isoelectric focusing. These solutions deal\nmainly with chaotropes and new detergents, which are both able to enhance\nprotein solubility. The input of these compounds in proteomics analysis of\nmembrane proteins is discussed, as well as future directions.",
"arxiv_id": "q-bio/0611077",
"authors": [
"Thierry Rabilloud",
"Sylvie Luche",
"V\u00e9ronique Santoni",
"Mireille Chevallet"
],
"categories": [
"q-bio.GN"
],
"journal_ref": "Methods Mol Biol 355 (2006) 111-20",
"title": "Detergents and Chaotropes for Protein Solubilization before Two-Dimensional Electrophoresis",
"url": "https://arxiv.org/abs/q-bio/0611077"
},
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