dorsal/arxiv
View SchemaImmunolocalization of Na+,K+-ATPase in the branchial cavity during the early development of the crayfish Astacus leptodactylus (Crustacea, Decapoda)
| Authors | Jean-Hervé Lignot, Gregorius Nugroho Susanto, Mireille Charmantier-Daures, Guy Charmantier |
|---|---|
| Categories | |
| ArXiv ID | q-bio/0611010 |
| URL | https://arxiv.org/abs/q-bio/0611010 |
| DOI | 10.1007/s00441-004-1015-2 |
| Journal | Cell Tissue Res 319 (2005) 331-339 |
Abstract
The ontogeny of osmoregulation was examined in the branchial cavity of embryonic and early post-embryonic stages of the crayfish Astacus leptodactylus maintained in freshwater, at the sub-cellular level through the detection of the sodium-potassium adenosine triphosphatase (Na(+),K(+)-ATPase). The embryonic rate of development was calculated according to the eye index (EI) which was 430-450 microm at hatching. The distribution of the enzyme was identified by immunofluorescence microscopy using a monoclonal antibody IgGalpha5 raised against the avian alpha-subunit of the Na(+),K(+)-ATPase. Immunoreactivity staining, indicating the presence of Na(+), K(+)-ATPase appeared in the gills of late embryos (EI>/=400 microm), i.e. a few days before hatching time, and steadily increased throughout the late embryonic and early post-embryonic development. The appearance of the enzyme correlates with the ability to osmoregulate which also occurs late in the embryonic development at EI 410-420 microm and with tissue differentiation within the gill filaments. These observations indicate that the physiological shift from osmoconforming embryos to hyper-regulating late embryos and post-hatching stages in freshwater must originate partly from the differentiation in the gill epithelia of ionocytes which are the site of ion pumping, as suggested by the location of Na(+),K(+)-ATPase. Only the gills were immunostained and a lack of specific staining was noted in the lamina and the branchiostegites. Therefore, osmoregulation through Na(+)active uptake is likely achieved in embryos at the gill level; all the newly formed gills in embryos function in ion regulation; other parts of the branchial chamber such as the branchiostegites and lamina do not appear to be involved in osmoregulation.
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"abstract": "The ontogeny of osmoregulation was examined in the branchial cavity of\nembryonic and early post-embryonic stages of the crayfish Astacus leptodactylus\nmaintained in freshwater, at the sub-cellular level through the detection of\nthe sodium-potassium adenosine triphosphatase (Na(+),K(+)-ATPase). The\nembryonic rate of development was calculated according to the eye index (EI)\nwhich was 430-450 microm at hatching. The distribution of the enzyme was\nidentified by immunofluorescence microscopy using a monoclonal antibody\nIgGalpha5 raised against the avian alpha-subunit of the Na(+),K(+)-ATPase.\nImmunoreactivity staining, indicating the presence of Na(+), K(+)-ATPase\nappeared in the gills of late embryos (EI\u003e/=400 microm), i.e. a few days before\nhatching time, and steadily increased throughout the late embryonic and early\npost-embryonic development. The appearance of the enzyme correlates with the\nability to osmoregulate which also occurs late in the embryonic development at\nEI 410-420 microm and with tissue differentiation within the gill filaments.\nThese observations indicate that the physiological shift from osmoconforming\nembryos to hyper-regulating late embryos and post-hatching stages in freshwater\nmust originate partly from the differentiation in the gill epithelia of\nionocytes which are the site of ion pumping, as suggested by the location of\nNa(+),K(+)-ATPase. Only the gills were immunostained and a lack of specific\nstaining was noted in the lamina and the branchiostegites. Therefore,\nosmoregulation through Na(+)active uptake is likely achieved in embryos at the\ngill level; all the newly formed gills in embryos function in ion regulation;\nother parts of the branchial chamber such as the branchiostegites and lamina do\nnot appear to be involved in osmoregulation.",
"arxiv_id": "q-bio/0611010",
"authors": [
"Jean-Herv\u00e9 Lignot",
"Gregorius Nugroho Susanto",
"Mireille Charmantier-Daures",
"Guy Charmantier"
],
"categories": [
"q-bio.PE"
],
"doi": "10.1007/s00441-004-1015-2",
"journal_ref": "Cell Tissue Res 319 (2005) 331-339",
"title": "Immunolocalization of Na+,K+-ATPase in the branchial cavity during the early development of the crayfish Astacus leptodactylus (Crustacea, Decapoda)",
"url": "https://arxiv.org/abs/q-bio/0611010"
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