dorsal/arxiv
View SchemaAlterations of the mitochondrial proteome caused by the absence of mitochondrial DNA: A proteomic view
| Authors | Mireille Chevallet, Pierre Lescuyer, Hélène Diemer, Alain van Dorsselaer, Emmanuelle Leize-Wagner, Thierry Rabilloud |
|---|---|
| Categories | |
| ArXiv ID | q-bio/0611081 |
| URL | https://arxiv.org/abs/q-bio/0611081 |
| DOI | 10.1002/elps.200500704 |
| Journal | Electrophoresis 27 (04/2006) 1574-83 |
Abstract
The proper functioning of mitochondria requires that both the mitochondrial and the nuclear genome are functional. To investigate the importance of the mitochondrial genome, which encodes only 13 subunits of the respiratory complexes, the mitochondrial rRNAs and a few tRNAs, we performed a comparative study on the 143B cell line and on its Rho-0 counterpart, i.e., devoid of mitochondrial DNA. Quantitative differences were found, of course in the respiratory complexes subunits, but also in the mitochondrial translation apparatus, mainly mitochondrial ribosomal proteins, and in the ion and protein import system, i.e., including membrane proteins. Various mitochondrial metabolic processes were also altered, especially electron transfer proteins and some dehydrogenases, but quite often on a few proteins for each pathway. This study also showed variations in some hypothetical or poorly characterized proteins, suggesting a mitochondrial localization for these proteins. Examples include a stomatin-like protein and a protein sharing homologies with bacterial proteins implicated in tyrosine catabolism. Proteins involved in apoptosis control are also found modulated in Rho-0 mitochondria.
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"abstract": "The proper functioning of mitochondria requires that both the mitochondrial\nand the nuclear genome are functional. To investigate the importance of the\nmitochondrial genome, which encodes only 13 subunits of the respiratory\ncomplexes, the mitochondrial rRNAs and a few tRNAs, we performed a comparative\nstudy on the 143B cell line and on its Rho-0 counterpart, i.e., devoid of\nmitochondrial DNA. Quantitative differences were found, of course in the\nrespiratory complexes subunits, but also in the mitochondrial translation\napparatus, mainly mitochondrial ribosomal proteins, and in the ion and protein\nimport system, i.e., including membrane proteins. Various mitochondrial\nmetabolic processes were also altered, especially electron transfer proteins\nand some dehydrogenases, but quite often on a few proteins for each pathway.\nThis study also showed variations in some hypothetical or poorly characterized\nproteins, suggesting a mitochondrial localization for these proteins. Examples\ninclude a stomatin-like protein and a protein sharing homologies with bacterial\nproteins implicated in tyrosine catabolism. Proteins involved in apoptosis\ncontrol are also found modulated in Rho-0 mitochondria.",
"arxiv_id": "q-bio/0611081",
"authors": [
"Mireille Chevallet",
"Pierre Lescuyer",
"H\u00e9l\u00e8ne Diemer",
"Alain van Dorsselaer",
"Emmanuelle Leize-Wagner",
"Thierry Rabilloud"
],
"categories": [
"q-bio.GN"
],
"doi": "10.1002/elps.200500704",
"journal_ref": "Electrophoresis 27 (04/2006) 1574-83",
"title": "Alterations of the mitochondrial proteome caused by the absence of mitochondrial DNA: A proteomic view",
"url": "https://arxiv.org/abs/q-bio/0611081"
},
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