dorsal/arxiv
View SchemaStudy the effects of metallic ions on the combination of DNA and histones with molecular combing technique
| Authors | Yu-Ying Liu, Peng-Ye Wang, Shuo-Xing Dou, Ping Xie, Wei-Chi Wang, Hua-Wei Yin |
|---|---|
| Categories | |
| ArXiv ID | q-bio/0503012 |
| URL | https://arxiv.org/abs/q-bio/0503012 |
| Journal | Chinese Science Bulletin, Vol.50, No. 8 (2005) 731-737 |
Abstract
The effects of monovalent (Na+, K+) and divalent (Mg2+, Ca2+, Mn2+) ions on the interaction between DNA and histone are studied using the molecular combing technique. Lamda-DNA molecules and DNA-histone complexes incubated with metal cations (Na+, K+, Mg2+, Ca2+, Mn2+) are stretched on hydrophobic surfaces, and directly observed by fluorescence microscopy. The results indicate that when these cations are added into the DNA solution, the fluorescence intensities of the stained DNA are reduced differently. The monovalent cations (Na+, K+) inhibit binding of histone to DNA. The divalent cations (Mg2+, Ca2+, Mn2+) enhance significantly the binding of histone to DNA and the binding of the DNA-histone complex to the hydrophobic surface. Mn2+ also induces condensation and aggregation of the DNA-histone complex.
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"abstract": "The effects of monovalent (Na+, K+) and divalent (Mg2+, Ca2+, Mn2+) ions on\nthe interaction between DNA and histone are studied using the molecular combing\ntechnique. Lamda-DNA molecules and DNA-histone complexes incubated with metal\ncations (Na+, K+, Mg2+, Ca2+, Mn2+) are stretched on hydrophobic surfaces, and\ndirectly observed by fluorescence microscopy. The results indicate that when\nthese cations are added into the DNA solution, the fluorescence intensities of\nthe stained DNA are reduced differently. The monovalent cations (Na+, K+)\ninhibit binding of histone to DNA. The divalent cations (Mg2+, Ca2+, Mn2+)\nenhance significantly the binding of histone to DNA and the binding of the\nDNA-histone complex to the hydrophobic surface. Mn2+ also induces condensation\nand aggregation of the DNA-histone complex.",
"arxiv_id": "q-bio/0503012",
"authors": [
"Yu-Ying Liu",
"Peng-Ye Wang",
"Shuo-Xing Dou",
"Ping Xie",
"Wei-Chi Wang",
"Hua-Wei Yin"
],
"categories": [
"q-bio.BM"
],
"journal_ref": "Chinese Science Bulletin, Vol.50, No. 8 (2005) 731-737",
"title": "Study the effects of metallic ions on the combination of DNA and histones with molecular combing technique",
"url": "https://arxiv.org/abs/q-bio/0503012"
},
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