dorsal/arxiv
View SchemaSucrose Solutions as Prospective Medium to Study the Vesicle Structure: SAXS and SANS study
| Authors | M. A. Kiselev, P. Lesieur, A. M. Kisselev, D. Lombardo, M. Killany, S. Lesieur |
|---|---|
| Categories | |
| ArXiv ID | physics/0110078 |
| URL | https://arxiv.org/abs/physics/0110078 |
| Journal | Journal of Alloys and Compounds v. 328 (2001) p. 71-76 |
Abstract
The possibility to use sucrose solutions as medium for X-ray and neutron small-angle scattering experiments has been explored for dimyristoylphosphatidylcholine (DMPC) vesicles and mixed DMPC/C_(12)E_(8) aggregates. The influence of sucrose concentration on phospholipid vesicles size and polydispersity has been investigated by complimentary X-ray and neutron scattering. Sucrose solutions decreased vesicle size and polydispersity and increased a contrast between phospholipid membrane and bulk solvent sufficiently for X-rays. 40% sucrose in H2O increased X-ray contrast by up to 10 times compared to pure H2O. The range of sucrose concentration 30%-40% created the best experimental conditions for the X-ray small-angle experiment with phospholipid vesicles.
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"abstract": "The possibility to use sucrose solutions as medium for X-ray and neutron\nsmall-angle scattering experiments has been explored for\ndimyristoylphosphatidylcholine (DMPC) vesicles and mixed DMPC/C_(12)E_(8)\naggregates. The influence of sucrose concentration on phospholipid vesicles\nsize and polydispersity has been investigated by complimentary X-ray and\nneutron scattering. Sucrose solutions decreased vesicle size and polydispersity\nand increased a contrast between phospholipid membrane and bulk solvent\nsufficiently for X-rays. 40% sucrose in H2O increased X-ray contrast by up to\n10 times compared to pure H2O. The range of sucrose concentration 30%-40%\ncreated the best experimental conditions for the X-ray small-angle experiment\nwith phospholipid vesicles.",
"arxiv_id": "physics/0110078",
"authors": [
"M. A. Kiselev",
"P. Lesieur",
"A. M. Kisselev",
"D. Lombardo",
"M. Killany",
"S. Lesieur"
],
"categories": [
"physics.bio-ph",
"cond-mat.soft",
"physics.chem-ph",
"q-bio"
],
"journal_ref": "Journal of Alloys and Compounds v. 328 (2001) p. 71-76",
"title": "Sucrose Solutions as Prospective Medium to Study the Vesicle Structure: SAXS and SANS study",
"url": "https://arxiv.org/abs/physics/0110078"
},
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