dorsal/arxiv
View SchemaA p53 Oscillator Model of DNA Break Repair Control
| Authors | Vijay Chickarmane, Ali Nadim, Animesh Ray, Herbert M. Sauro |
|---|---|
| Categories | |
| ArXiv ID | q-bio/0510002 |
| URL | https://arxiv.org/abs/q-bio/0510002 |
Abstract
The transcription factor p53 is an important regulator of cell fate. Mutations in p53 gene are associated with many cancers. In response to signals such as DNA damage, p53 controls the transcription of a series of genes that cause cell cycle arrest during which DNA damage is repaired, or triggers programmed cell death that eliminates possibly cancerous cells wherein DNA damage might have remained unrepaired. Previous experiments showed oscillations in p53 level in response to DNA damage, but the mechanism of oscillation remained unclear. Here we examine a model where the concentrations of p53 isoforms are regulated by Mdm22, Arf, Siah, and beta-catenin. The extent of DNA damage is signalled through the switch-like activity of a DNA damage sensor, the DNA-dependent protein kinase Atm. This switch is responsible for initiating and terminating oscillations in p53 concentration. The strength of the DNA damage signal modulates the number of oscillatory waves of p53 and Mdm22 but not the frequency or amplitude of oscillations{a result that recapitulates experimental findings. A critical fnding was that the phosphorylated form of Nbs11, a member of the DNA break repair complex Mre11-Rad50-Nbs11 (MRN), must augment the activity of Atm kinase. While there is in vitro support for this assumption, this activity appears essential for p53 dynamics. The model provides several predictions concerning, among others, degradation of the phosphorylated form of p53, the rate of DNA repair as a function of DNA damage, the sensitivity of p53 oscillation to transcription rates of SIAH, beta-CATENIN and ARF, and the hysteretic behavior of active Atm kinase levels with respect to the DNA damage signal
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"abstract": "The transcription factor p53 is an important regulator of cell fate.\nMutations in p53 gene are associated with many cancers. In response to signals\nsuch as DNA damage, p53 controls the transcription of a series of genes that\ncause cell cycle arrest during which DNA damage is repaired, or triggers\nprogrammed cell death that eliminates possibly cancerous cells wherein DNA\ndamage might have remained unrepaired. Previous experiments showed oscillations\nin p53 level in response to DNA damage, but the mechanism of oscillation\nremained unclear. Here we examine a model where the concentrations of p53\nisoforms are regulated by Mdm22, Arf, Siah, and beta-catenin. The extent of DNA\ndamage is signalled through the switch-like activity of a DNA damage sensor,\nthe DNA-dependent protein kinase Atm. This switch is responsible for initiating\nand terminating oscillations in p53 concentration. The strength of the DNA\ndamage signal modulates the number of oscillatory waves of p53 and Mdm22 but\nnot the frequency or amplitude of oscillations{a result that recapitulates\nexperimental findings. A critical fnding was that the phosphorylated form of\nNbs11, a member of the DNA break repair complex Mre11-Rad50-Nbs11 (MRN), must\naugment the activity of Atm kinase. While there is in vitro support for this\nassumption, this activity appears essential for p53 dynamics. The model\nprovides several predictions concerning, among others, degradation of the\nphosphorylated form of p53, the rate of DNA repair as a function of DNA damage,\nthe sensitivity of p53 oscillation to transcription rates of SIAH, beta-CATENIN\nand ARF, and the hysteretic behavior of active Atm kinase levels with respect\nto the DNA damage signal",
"arxiv_id": "q-bio/0510002",
"authors": [
"Vijay Chickarmane",
"Ali Nadim",
"Animesh Ray",
"Herbert M. Sauro"
],
"categories": [
"q-bio.MN"
],
"title": "A p53 Oscillator Model of DNA Break Repair Control",
"url": "https://arxiv.org/abs/q-bio/0510002"
},
"schema_id": "dorsal/arxiv",
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"type": "Model",
"variant": "snapshot-2026-03-01",
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