dorsal/arxiv
View SchemaThe effect of genome length on ejection forces in bacteriophage lambda
| Authors | Paul Grayson, Alex Evilevitch, Mandar M. Inamdar, Prashant K. Purohit, William M. Gelbart, Charles M. Knobler, Rob Phillips |
|---|---|
| Categories | |
| ArXiv ID | q-bio/0505042 |
| URL | https://arxiv.org/abs/q-bio/0505042 |
Abstract
A variety of viruses tightly pack their genetic material into protein capsids that are barely large enough to enclose the genome. In particular, in bacteriophages, forces as high as 60 pN are encountered during packaging and ejection, produced by DNA bending elasticity and self-interactions. The high forces are believed to be important for the ejection process, though the extent of their involvement is not yet clear. As a result, there is a need for quantitative models and experiments that reveal the nature of the forces relevant to DNA ejection. Here we report measurements of the ejection forces for two different mutants of bacteriophage lambda, lambda b221cI26 and lambda cI60, which differ in genome length by ~30%. As expected for a force-driven ejection mechanism, the osmotic pressure at which DNA release is completely inhibited varies with the genome length: we find inhibition pressures of 15 atm and 25 atm, respectively, values that are in agreement with our theoretical calculations.
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"abstract": "A variety of viruses tightly pack their genetic material into protein capsids\nthat are barely large enough to enclose the genome. In particular, in\nbacteriophages, forces as high as 60 pN are encountered during packaging and\nejection, produced by DNA bending elasticity and self-interactions. The high\nforces are believed to be important for the ejection process, though the extent\nof their involvement is not yet clear. As a result, there is a need for\nquantitative models and experiments that reveal the nature of the forces\nrelevant to DNA ejection. Here we report measurements of the ejection forces\nfor two different mutants of bacteriophage lambda, lambda b221cI26 and lambda\ncI60, which differ in genome length by ~30%. As expected for a force-driven\nejection mechanism, the osmotic pressure at which DNA release is completely\ninhibited varies with the genome length: we find inhibition pressures of 15 atm\nand 25 atm, respectively, values that are in agreement with our theoretical\ncalculations.",
"arxiv_id": "q-bio/0505042",
"authors": [
"Paul Grayson",
"Alex Evilevitch",
"Mandar M. Inamdar",
"Prashant K. Purohit",
"William M. Gelbart",
"Charles M. Knobler",
"Rob Phillips"
],
"categories": [
"q-bio.BM"
],
"title": "The effect of genome length on ejection forces in bacteriophage lambda",
"url": "https://arxiv.org/abs/q-bio/0505042"
},
"schema_id": "dorsal/arxiv",
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