dorsal/arxiv
View SchemaEvaluation of cell wall preparations for proteomics: a new procedure for purifying cell walls from Arabidopsis hypocotyls
| Authors | Leila Feiz, Muhammad Irshad, Rafael F Pont-Lezica, Hervé Canut, Elisabeth Jamet |
|---|---|
| Categories | |
| ArXiv ID | q-bio/0610022 |
| URL | https://arxiv.org/abs/q-bio/0610022 |
| DOI | 10.1186/1746-4811-2-10 |
| Journal | Plant Methods 2 (2006) 10 |
Abstract
The ultimate goal of proteomic analysis of a cell compartment should be the exhaustive identification of resident proteins; excluding proteins from other cell compartments. Plant cell walls possess specific difficulties. Several reported procedures to isolate cell walls for proteomic analyses led to the isolation of a high proportion (more than 50%) of predicted intracellular proteins. The rationales of several published procedures to isolate cell walls for proteomics were analyzed, with regard to the bioinformatic-predicted subcellular localization of the identified proteins. A new procedure was developed to prepare cell walls from etiolated hypocotyls of Arabidopsis thaliana. After salt extraction, a high proportion of proteins predicted to be secreted was released (73%), belonging to the same functional classes as proteins identified using previously described protocols. The new cell wall preparation described in this paper gives the lowest proportion of proteins predicted to be intracellular when compared to available protocols. The application of its principles should lead to a more realistic view of the cell wall proteome, at least for the weakly bound CWP extractable by salts. In addition, it offers a clean cell wall preparation for subsequent extraction of strongly bound CWP.
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"abstract": "The ultimate goal of proteomic analysis of a cell compartment should be the\nexhaustive identification of resident proteins; excluding proteins from other\ncell compartments. Plant cell walls possess specific difficulties. Several\nreported procedures to isolate cell walls for proteomic analyses led to the\nisolation of a high proportion (more than 50%) of predicted intracellular\nproteins. The rationales of several published procedures to isolate cell walls\nfor proteomics were analyzed, with regard to the bioinformatic-predicted\nsubcellular localization of the identified proteins. A new procedure was\ndeveloped to prepare cell walls from etiolated hypocotyls of Arabidopsis\nthaliana. After salt extraction, a high proportion of proteins predicted to be\nsecreted was released (73%), belonging to the same functional classes as\nproteins identified using previously described protocols. The new cell wall\npreparation described in this paper gives the lowest proportion of proteins\npredicted to be intracellular when compared to available protocols. The\napplication of its principles should lead to a more realistic view of the cell\nwall proteome, at least for the weakly bound CWP extractable by salts. In\naddition, it offers a clean cell wall preparation for subsequent extraction of\nstrongly bound CWP.",
"arxiv_id": "q-bio/0610022",
"authors": [
"Leila Feiz",
"Muhammad Irshad",
"Rafael F Pont-Lezica",
"Herv\u00e9 Canut",
"Elisabeth Jamet"
],
"categories": [
"q-bio.GN"
],
"doi": "10.1186/1746-4811-2-10",
"journal_ref": "Plant Methods 2 (2006) 10",
"title": "Evaluation of cell wall preparations for proteomics: a new procedure for purifying cell walls from Arabidopsis hypocotyls",
"url": "https://arxiv.org/abs/q-bio/0610022"
},
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