dorsal/arxiv
View SchemaA Sucrose Solution Application to the Study of Model Biological Membranes
| Authors | M. A. Kiselev, P. Lesieur, A. M. Kisselev, D. Lombardo, M. Killany, S. Lesieur, M. Ollivon |
|---|---|
| Categories | |
| ArXiv ID | physics/0110079 |
| URL | https://arxiv.org/abs/physics/0110079 |
| DOI | 10.1016/S0168-9002(01)01087-7 |
| Journal | Nuclear Instruments & Methods in Physics Research, vol. A 470 (2001) p. 409-416 |
Abstract
The small-angle X-ray and neutron scattering, time resolved X-ray small-angle and wide-angle diffraction coupled with differential scanning calorimetry have been applied to the investigation of unilamellar and multilamellar dimyristoylphosphatidylcholine (DMPC) vesicles in sucrose buffers with sucrose concentrations from 0 to 60%. Sucrose buffer decreased vesicle size and polydispersity and increased an X-ray contrast between phospholipid membrane and bulk solvent sufficiently. No influence of sucrose on the membrane thickness or mutual packing of hydrocarbon chains has been detected. The region of sucrose concentrations 30%-40% created the best experimental conditions for X-ray small-angle experiments with phospholipid vesicles.
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"abstract": "The small-angle X-ray and neutron scattering, time resolved X-ray small-angle\nand wide-angle diffraction coupled with differential scanning calorimetry have\nbeen applied to the investigation of unilamellar and multilamellar\ndimyristoylphosphatidylcholine (DMPC) vesicles in sucrose buffers with sucrose\nconcentrations from 0 to 60%. Sucrose buffer decreased vesicle size and\npolydispersity and increased an X-ray contrast between phospholipid membrane\nand bulk solvent sufficiently. No influence of sucrose on the membrane\nthickness or mutual packing of hydrocarbon chains has been detected. The region\nof sucrose concentrations 30%-40% created the best experimental conditions for\nX-ray small-angle experiments with phospholipid vesicles.",
"arxiv_id": "physics/0110079",
"authors": [
"M. A. Kiselev",
"P. Lesieur",
"A. M. Kisselev",
"D. Lombardo",
"M. Killany",
"S. Lesieur",
"M. Ollivon"
],
"categories": [
"physics.bio-ph",
"cond-mat.soft",
"physics.chem-ph",
"q-bio"
],
"doi": "10.1016/S0168-9002(01)01087-7",
"journal_ref": "Nuclear Instruments \u0026 Methods in Physics Research, vol. A 470\n (2001) p. 409-416",
"title": "A Sucrose Solution Application to the Study of Model Biological Membranes",
"url": "https://arxiv.org/abs/physics/0110079"
},
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