dorsal/arxiv
View SchemaCombined surface acoustic wave and surface plasmon resonance measurement of collagen and fibrinogen layers
| Authors | Jean-Michel Friedt, Laurent Francis |
|---|---|
| Categories | |
| ArXiv ID | physics/0310096 |
| URL | https://arxiv.org/abs/physics/0310096 |
| License | http://arxiv.org/licenses/nonexclusive-distrib/1.0/ |
Abstract
We use an instrument combining optical (surface plasmon resonance) and acoustic (Love mode acoustic wave device) real-time measurements on a same surface for the identification of water content in collagen and fibrinogen protein layers. After calibration of the surface acoustic wave device sensitivity by copper electrodeposition, the bound mass and its physical properties -- density and optical index -- are extracted from the complementary measurement techniques and lead to thickness and water ratio values compatible with the observed signal shifts. Such results are especially usefully for protein layers with a high water content as shown here for collagen on an hydrophobic surface. We obtain the following results: collagen layers include 70+/-20 % water and are 16+/-3 to 19+/-3 nm thick for bulk concentrations ranging from 30 to 300 ug/ml. Fibrinogen layers include 50+/-10 % water for layer thicknesses in the 6+/-1.5 to 13+/-2 nm range when the bulk concentration is in the 46 to 460 ug/ml range.
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"date_created": "2026-03-02T18:00:46.237000Z",
"date_modified": "2026-03-02T18:00:46.237000Z",
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"abstract": "We use an instrument combining optical (surface plasmon resonance) and\nacoustic (Love mode acoustic wave device) real-time measurements on a same\nsurface for the identification of water content in collagen and fibrinogen\nprotein layers. After calibration of the surface acoustic wave device\nsensitivity by copper electrodeposition, the bound mass and its physical\nproperties -- density and optical index -- are extracted from the complementary\nmeasurement techniques and lead to thickness and water ratio values compatible\nwith the observed signal shifts. Such results are especially usefully for\nprotein layers with a high water content as shown here for collagen on an\nhydrophobic surface. We obtain the following results: collagen layers include\n70+/-20 % water and are 16+/-3 to 19+/-3 nm thick for bulk concentrations\nranging from 30 to 300 ug/ml. Fibrinogen layers include 50+/-10 % water for\nlayer thicknesses in the 6+/-1.5 to 13+/-2 nm range when the bulk concentration\nis in the 46 to 460 ug/ml range.",
"arxiv_id": "physics/0310096",
"authors": [
"Jean-Michel Friedt",
"Laurent Francis"
],
"categories": [
"physics.bio-ph",
"cond-mat.soft",
"q-bio.QM"
],
"license": "http://arxiv.org/licenses/nonexclusive-distrib/1.0/",
"title": "Combined surface acoustic wave and surface plasmon resonance measurement of collagen and fibrinogen layers",
"url": "https://arxiv.org/abs/physics/0310096"
},
"schema_id": "dorsal/arxiv",
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