{
  "annotation_id": "76b7a7b2-7d10-4197-86c9-3d78edfd6d22",
  "date_created": "2026-03-02T18:01:28.593000Z",
  "date_modified": "2026-03-02T18:01:28.593000Z",
  "file_hash": "6c683dc5aae79ef9828e0a2a21b04a47999cbf30f89aac6b6c288177c490e666",
  "private": false,
  "record": {
    "abstract": "We report the development and detailed calibration of a multiphoton\nfluorescence lifetime imaging system (FLIM) using a streak camera. The present\nsystem is versatile with high spatial (0.2 micron) and temporal (50 psec)\nresolution and allows rapid data acquisition and reliable and reproducible\nlifetime determinations. The system was calibrated with standard fluorescent\ndyes and the lifetime values obtained were in very good agreement with values\nreported in literature for these dyes. We also demonstrate the applicability of\nthe system to FLIM studies in cellular specimens including stained pollen\ngrains and fibroblast cells expressing green fluorescent protein. The lifetime\nvalues obtained matched well with those reported earlier by other groups for\nthese same specimens. Potential applications of the present system include the\nmeasurement of intracellular physiology and Fluorescence Resonance Energy\nTransfer (FRET) imaging which are discussed in the context of live cell\nimaging.",
    "arxiv_id": "q-bio/0309021",
    "authors": [
      "R. V. Krishnan",
      "H. Saitoh",
      "H. Terada",
      "V. E. Centonze",
      "B. Herman"
    ],
    "categories": [
      "q-bio.QM"
    ],
    "doi": "10.1063/1.1569410",
    "journal_ref": "Review of Scientific Instruments, Vol.74, 2714 (May 2003)",
    "title": "Development of a Multiphoton Fluorescence Lifetime Imaging Microscopy   (FLIM) system using a Streak Camera",
    "url": "https://arxiv.org/abs/q-bio/0309021"
  },
  "schema_id": "dorsal/arxiv",
  "source": {
    "execution_id": "9382edaa-c022-43bc-9855-1cf3921d463f",
    "id": "arXiv Dataset IDs",
    "type": "Model",
    "variant": "snapshot-2026-03-01",
    "version": "0.1.0"
  },
  "user_id": 1000002
}