dorsal/arxiv
View SchemaMicro-Bioreactor Mimicking a Cellular Environment
| Authors | Masahiko Hase, Ayako Yamada, Tsutomu Hamada, Kenichi Yoshikawa |
|---|---|
| Categories | |
| ArXiv ID | physics/0605145 |
| URL | https://arxiv.org/abs/physics/0605145 |
Abstract
We report that a cell-sized water droplet (CWD) with a diameter of several tens of microns can serve as a micro-biochemical reactor. Since the droplet inner surface is covered by a phospholipid layer, it provides an environment similar to that in living cells. The CWD is manipulated with laser tweezers and we monitor the time-development of biochemical reactions in a single CWD by fusing two droplets which contain a substrate and an enzyme, respectively. As actual examples of enzymatic reactions, we present results on the reaction of calcein with esterase and on the expression reaction of green fluorescent protein.
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"abstract": "We report that a cell-sized water droplet (CWD) with a diameter of several\ntens of microns can serve as a micro-biochemical reactor. Since the droplet\ninner surface is covered by a phospholipid layer, it provides an environment\nsimilar to that in living cells. The CWD is manipulated with laser tweezers and\nwe monitor the time-development of biochemical reactions in a single CWD by\nfusing two droplets which contain a substrate and an enzyme, respectively. As\nactual examples of enzymatic reactions, we present results on the reaction of\ncalcein with esterase and on the expression reaction of green fluorescent\nprotein.",
"arxiv_id": "physics/0605145",
"authors": [
"Masahiko Hase",
"Ayako Yamada",
"Tsutomu Hamada",
"Kenichi Yoshikawa"
],
"categories": [
"physics.bio-ph"
],
"title": "Micro-Bioreactor Mimicking a Cellular Environment",
"url": "https://arxiv.org/abs/physics/0605145"
},
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