{
  "annotation_id": "6c5b6cd3-8cce-40e9-98b2-83bb94210888",
  "date_created": "2026-03-02T18:01:35.444000Z",
  "date_modified": "2026-03-02T18:01:35.444000Z",
  "file_hash": "355557afca464d8a0e1d2130d0ff60e3b62ccd00eecf5b9b1e282aeca109846f",
  "private": false,
  "record": {
    "abstract": "Integrating biological information from different sources to understand\ncellular processes is an important problem in systems biology. We use data from\nmRNA expression arrays and chemical kinetics to formulate a metabolic model\nrelevant to K562 erythroleukemia cells. MAP kinase pathway activation alters\nthe expression of metabolic enzymes in K562 cells. Our array data show changes\nin expression of lactate dehydrogenase (LDH) isoforms after treatment with\nphorbol 12-myristate 13-acetate (PMA), which activates MAP kinase signaling. We\nmodel the change in lactate production which occurs when the MAP kinase pathway\nis activated, using a non-equilibrium, chemical-kinetic model of homolactic\nfermentation. In particular, we examine the role of LDH isoforms, which\ncatalyze the conversion of pyruvate to lactate. Changes in the isoform ratio\nare not the primary determinant of the production of lactate. Rather, the total\nconcentration of LDH controls the lactate concentration.",
    "arxiv_id": "q-bio/0511013",
    "authors": [
      "Joshua Downer",
      "Joel R. Sevinsky",
      "Natalie G. Ahn",
      "Katheryn A. Resing",
      "M. D. Betterton"
    ],
    "categories": [
      "q-bio.SC",
      "q-bio.QM"
    ],
    "doi": "10.1016/j.jtbi.2005.10.007",
    "journal_ref": "J Theor Biol 240: 464-474 (2006)",
    "title": "Incorporating expression data in metabolic modeling: a case study of   lactate dehydrogenase",
    "url": "https://arxiv.org/abs/q-bio/0511013"
  },
  "schema_id": "dorsal/arxiv",
  "source": {
    "execution_id": "5c0e2721-8026-4143-ad3f-314b7d611fd1",
    "id": "arXiv Dataset IDs",
    "type": "Model",
    "variant": "snapshot-2026-03-01",
    "version": "0.1.0"
  },
  "user_id": 1000002
}