dorsal/arxiv
View SchemaWhat can one learn from two-state single molecule trajectories?
| Authors | Ophir Flomenbom, Joseph Klafter, Attila Szabo |
|---|---|
| Categories | |
| ArXiv ID | q-bio/0502006 |
| URL | https://arxiv.org/abs/q-bio/0502006 |
| DOI | 10.1529/biophysj.104.055905 |
| Journal | Biophys. J. 88, 3780-3783 (2005) |
Abstract
A time trajectory of an observable that fluctuates between two values (say, on and off), stemming from some unknown multi-substate kinetic scheme, is the output of many single molecule experiments. Here we show that when all successive waiting times along the trajectory are uncorrelated the on and the off waiting time probability density functions (PDFs) contain all the information. By relating the lack of correlation in the trajectory to the topology of kinetic schemes, we can immediately specify those kinetic schemes that are equally consistent with experiment, which means that it is impossible to differentiate between them by any sophisticated analyses of the trajectory. Correlated trajectories, however, contain additional information about the underlying kinetic scheme, and we consider the strategy that one should use to extract it. An example is given on correlations in the activity of individual lipase molecules.
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"abstract": "A time trajectory of an observable that fluctuates between two values (say,\non and off), stemming from some unknown multi-substate kinetic scheme, is the\noutput of many single molecule experiments. Here we show that when all\nsuccessive waiting times along the trajectory are uncorrelated the on and the\noff waiting time probability density functions (PDFs) contain all the\ninformation. By relating the lack of correlation in the trajectory to the\ntopology of kinetic schemes, we can immediately specify those kinetic schemes\nthat are equally consistent with experiment, which means that it is impossible\nto differentiate between them by any sophisticated analyses of the trajectory.\nCorrelated trajectories, however, contain additional information about the\nunderlying kinetic scheme, and we consider the strategy that one should use to\nextract it. An example is given on correlations in the activity of individual\nlipase molecules.",
"arxiv_id": "q-bio/0502006",
"authors": [
"Ophir Flomenbom",
"Joseph Klafter",
"Attila Szabo"
],
"categories": [
"q-bio.SC",
"q-bio.QM"
],
"doi": "10.1529/biophysj.104.055905",
"journal_ref": "Biophys. J. 88, 3780-3783 (2005)",
"title": "What can one learn from two-state single molecule trajectories?",
"url": "https://arxiv.org/abs/q-bio/0502006"
},
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