dorsal/arxiv
View SchemaForced-unfolding and force-quench refolding of RNA hairpins
| Authors | Changbong Hyeon, D. Thirumalai |
|---|---|
| Categories | |
| ArXiv ID | q-bio/0601043 |
| URL | https://arxiv.org/abs/q-bio/0601043 |
| DOI | 10.1529/biophysj.105.078030 |
| Journal | Biophys. J. (2006) 80 3410-3427 |
Abstract
Using coarse-grained model we have explored forced-unfolding of RNA hairpin as a function of $f_S$ and the loading rate ($r_f$). The simulations and theoretical analysis have been done without and with the handles that are explicitly modeled by semiflexible polymer chains. The mechanisms and time scales for denaturation by temperature jump and mechanical unfolding are vastly different. The directed perturbation of the native state by $f_S$ results in a sequential unfolding of the hairpin starting from their ends whereas thermal denaturation occurs stochastically. From the dependence of the unfolding rates on $r_f$ and $f_S$ we show that the position of the unfolding transition state (TS) is not a constant but moves dramatically as either $r_f$ or $f_S$ is changed. The TS movements are interpreted by adopting the Hammond postulate for forced-unfolding. Forced-unfolding simulations of RNA, with handles attached to the two ends, show that the value of the unfolding force increases (especially at high pulling speeds) as the length of the handles increases. The pathways for refolding of RNA from stretched initial conformation, upon quenching $f_S$ to the quench force $f_Q$, are highly heterogeneous. The refolding times, upon force quench, are at least an order of magnitude greater than those obtained by temperature quench. The long $f_Q$-dependent refolding times starting from fully stretched states are analyzed using a model that accounts for the microscopic steps in the rate limiting step which involves the trans to gauche transitions of the dihedral angles in the GAAA tetraloop. The simulations with explicit molecular model for the handles show that the dynamics of force-quench refolding is strongly dependent on the interplay of their contour length and the persistence length, and the RNA persistence length.
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"abstract": "Using coarse-grained model we have explored forced-unfolding of RNA hairpin\nas a function of $f_S$ and the loading rate ($r_f$). The simulations and\ntheoretical analysis have been done without and with the handles that are\nexplicitly modeled by semiflexible polymer chains. The mechanisms and time\nscales for denaturation by temperature jump and mechanical unfolding are vastly\ndifferent. The directed perturbation of the native state by $f_S$ results in a\nsequential unfolding of the hairpin starting from their ends whereas thermal\ndenaturation occurs stochastically. From the dependence of the unfolding rates\non $r_f$ and $f_S$ we show that the position of the unfolding transition state\n(TS) is not a constant but moves dramatically as either $r_f$ or $f_S$ is\nchanged. The TS movements are interpreted by adopting the Hammond postulate for\nforced-unfolding. Forced-unfolding simulations of RNA, with handles attached to\nthe two ends, show that the value of the unfolding force increases (especially\nat high pulling speeds) as the length of the handles increases. The pathways\nfor refolding of RNA from stretched initial conformation, upon quenching $f_S$\nto the quench force $f_Q$, are highly heterogeneous. The refolding times, upon\nforce quench, are at least an order of magnitude greater than those obtained by\ntemperature quench. The long $f_Q$-dependent refolding times starting from\nfully stretched states are analyzed using a model that accounts for the\nmicroscopic steps in the rate limiting step which involves the trans to gauche\ntransitions of the dihedral angles in the GAAA tetraloop. The simulations with\nexplicit molecular model for the handles show that the dynamics of force-quench\nrefolding is strongly dependent on the interplay of their contour length and\nthe persistence length, and the RNA persistence length.",
"arxiv_id": "q-bio/0601043",
"authors": [
"Changbong Hyeon",
"D. Thirumalai"
],
"categories": [
"q-bio.BM",
"cond-mat.soft"
],
"doi": "10.1529/biophysj.105.078030",
"journal_ref": "Biophys. J. (2006) 80 3410-3427",
"title": "Forced-unfolding and force-quench refolding of RNA hairpins",
"url": "https://arxiv.org/abs/q-bio/0601043"
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