dorsal/arxiv
View SchemaThermodynamic and Kinetic Analysis of Sensitivity Amplification in Biological Signal Transduction
| Authors | Hong Qian |
|---|---|
| Categories | |
| ArXiv ID | physics/0207049 |
| URL | https://arxiv.org/abs/physics/0207049 |
| DOI | 10.1016/S0301-4622(03)00068-1 |
| Journal | Biophysical Chemistry, Vol. 105, pp. 585-593 (2003) |
Abstract
Based on a thermodynamic analysis of the kinetic model for the protein phosphorylation-dephosphorylation cycle, we study the ATP (or GTP) energy utilization of this ubiquitous biological signal transduction process. It is shown that the free energy from hydrolysis inside cells, $\Delta G$ (phosphorylation potential), controls the amplification and sensitivity of the switch-like cellular module; the response coefficient of the sensitivity amplification approaches the optimal 1 and the Hill coefficient increases with increasing $\Delta G$. We discover that zero-order ultrasensitivity is mathematically equivalent to allosteric cooperativity. Furthermore, we show that the high amplification in ultrasensitivity is mechanistically related to the proofreading kinetics for protein biosynthesis. Both utilize multiple kinetic cycles in time to gain temporal cooperativity, in contrast to allosteric cooperativity that utilizes multiple subunits in a protein.
{
"annotation_id": "5b1b12fe-901a-4af7-8fe7-3c3fb05e047e",
"date_created": "2026-03-02T18:00:39.474000Z",
"date_modified": "2026-03-02T18:00:39.474000Z",
"file_hash": "75775ef638bed229395dd33f03c84583dc0598227274bb3452d9dbd83cbd978f",
"private": false,
"record": {
"abstract": "Based on a thermodynamic analysis of the kinetic model for the protein\nphosphorylation-dephosphorylation cycle, we study the ATP (or GTP) energy\nutilization of this ubiquitous biological signal transduction process. It is\nshown that the free energy from hydrolysis inside cells, $\\Delta G$\n(phosphorylation potential), controls the amplification and sensitivity of the\nswitch-like cellular module; the response coefficient of the sensitivity\namplification approaches the optimal 1 and the Hill coefficient increases with\nincreasing $\\Delta G$. We discover that zero-order ultrasensitivity is\nmathematically equivalent to allosteric cooperativity. Furthermore, we show\nthat the high amplification in ultrasensitivity is mechanistically related to\nthe proofreading kinetics for protein biosynthesis. Both utilize multiple\nkinetic cycles in time to gain temporal cooperativity, in contrast to\nallosteric cooperativity that utilizes multiple subunits in a protein.",
"arxiv_id": "physics/0207049",
"authors": [
"Hong Qian"
],
"categories": [
"physics.bio-ph",
"q-bio"
],
"doi": "10.1016/S0301-4622(03)00068-1",
"journal_ref": "Biophysical Chemistry, Vol. 105, pp. 585-593 (2003)",
"title": "Thermodynamic and Kinetic Analysis of Sensitivity Amplification in Biological Signal Transduction",
"url": "https://arxiv.org/abs/physics/0207049"
},
"schema_id": "dorsal/arxiv",
"source": {
"execution_id": "478aa3d5-a607-4018-ba7d-524de74d2f63",
"id": "arXiv Dataset IDs",
"type": "Model",
"variant": "snapshot-2026-03-01",
"version": "0.1.0"
},
"user_id": 1000002
}