dorsal/arxiv
View SchemaMeasurements of Multicomponent Diffusion Coefficients for Lysozyme Chloride in Water and Aqueous Na$_2$SO$_4$
| Authors | D. Buzatu, E. Petrescu, F. D. Buzatu, J. G. Albright |
|---|---|
| Categories | |
| ArXiv ID | physics/0403099 |
| URL | https://arxiv.org/abs/physics/0403099 |
Abstract
This paper presents a diffusion experimental study for ternary lysozyme-Na$_2$SO$_4$-water system, from moderate precipitant concentrations into the supersaturated region and provides a complete set of four diffusion coefficients. These data are important in order to provide accurate models of protein diffusion with applications in growth of protein crystals for X-ray diffraction studies. All three-component mutual-diffusion experiments reported here were performed by Rayleigh interferometry at pH$=4.5$, T$=25^o$ C and at a mean lysozyme concentration (average of top and bottom solution concentrations) of 0.6 mM (8.6 mg/mL). Four experiments, with different combinations of protein and Na$_2$SO$_4$ concentration differences, were performed at each of five mean Na$_2$SO$_4$ concentrations (0.1, 0.25, 0.5, 0.65 and 0.8 M), for a total of 20 experiments. In addition, we have measured dynamic light-scattering diffusion coefficients of the ternary system lysozyme chloride-Na$_2$SO$_4$-water.
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"abstract": "This paper presents a diffusion experimental study for ternary\nlysozyme-Na$_2$SO$_4$-water system, from moderate precipitant concentrations\ninto the supersaturated region and provides a complete set of four diffusion\ncoefficients. These data are important in order to provide accurate models of\nprotein diffusion with applications in growth of protein crystals for X-ray\ndiffraction studies. All three-component mutual-diffusion experiments reported\nhere were performed by Rayleigh interferometry at pH$=4.5$, T$=25^o$ C and at a\nmean lysozyme concentration (average of top and bottom solution concentrations)\nof 0.6 mM (8.6 mg/mL). Four experiments, with different combinations of protein\nand Na$_2$SO$_4$ concentration differences, were performed at each of five mean\nNa$_2$SO$_4$ concentrations (0.1, 0.25, 0.5, 0.65 and 0.8 M), for a total of 20\nexperiments. In addition, we have measured dynamic light-scattering diffusion\ncoefficients of the ternary system lysozyme chloride-Na$_2$SO$_4$-water.",
"arxiv_id": "physics/0403099",
"authors": [
"D. Buzatu",
"E. Petrescu",
"F. D. Buzatu",
"J. G. Albright"
],
"categories": [
"physics.chem-ph"
],
"title": "Measurements of Multicomponent Diffusion Coefficients for Lysozyme Chloride in Water and Aqueous Na$_2$SO$_4$",
"url": "https://arxiv.org/abs/physics/0403099"
},
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