dorsal/arxiv
View SchemaX-ray microanalysis in STEM of short-term physico-chemical reactions at bioactive glass particles / biological fluids interface. Determination of O/Si atomic ratios
| Authors | V. Blanchet, E. Jallot, J. Michel, L. Wortham, D. Laurent-Maquin, G. Balossier |
|---|---|
| Categories | |
| ArXiv ID | physics/0412146 |
| URL | https://arxiv.org/abs/physics/0412146 |
| Journal | Surface and Interface Analysis 36 (2004) 658-665 |
Abstract
Short-term physico-chemical reactions at the interface between bioactive glass particles and biological fluids are studied and we focus our attention on the measurements of O/Si atomic ratio. The studied bioactive glass is in the SiO2-Na2O-CaO-P2O5-K2O-Al2O3-MgO system. The elemental analysis is performed at the submicrometer scale by STEM associated with EDXS and EELS. We previously developed an EDXS quantification method based on the ratio method and taking into account local absorption corrections. In this way, we use EELS data to determine, by an iterative process, the local mass thickness which is an essential parameter to correct absorption in EDXS spectra. After different delays of immersion of bioactive glass particles in a simulated biological solution, results show the formation of different surface layers at the bioactive glass periphery. Before one day of immersion, we observe the presence of an already shown (Si,O,Al) rich layer at the periphery. In this paper, we demonstrate that a thin electron dense (Si,O) layer is formed on top of the (Si,O,Al) layer. In this (Si,O) layer, depleted in aluminium, we point out an increase of oxygen weight concentration which can be interpreted by the presence of Si(OH)4 groups, that permit the formation of a (Ca,P) layer. Aluminium plays a role in the glass solubility and may inhibit apatite nucleation. After the beginning of the (Ca,P) layer formation, the size of the electron dense (Si,O) layer decreases and tends to disappear. After two days of immersion, the (Ca,P) layer grows in thickness and leads to apatite precipitation
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"abstract": "Short-term physico-chemical reactions at the interface between bioactive\nglass particles and biological fluids are studied and we focus our attention on\nthe measurements of O/Si atomic ratio. The studied bioactive glass is in the\nSiO2-Na2O-CaO-P2O5-K2O-Al2O3-MgO system. The elemental analysis is performed at\nthe submicrometer scale by STEM associated with EDXS and EELS. We previously\ndeveloped an EDXS quantification method based on the ratio method and taking\ninto account local absorption corrections. In this way, we use EELS data to\ndetermine, by an iterative process, the local mass thickness which is an\nessential parameter to correct absorption in EDXS spectra. After different\ndelays of immersion of bioactive glass particles in a simulated biological\nsolution, results show the formation of different surface layers at the\nbioactive glass periphery. Before one day of immersion, we observe the presence\nof an already shown (Si,O,Al) rich layer at the periphery. In this paper, we\ndemonstrate that a thin electron dense (Si,O) layer is formed on top of the\n(Si,O,Al) layer. In this (Si,O) layer, depleted in aluminium, we point out an\nincrease of oxygen weight concentration which can be interpreted by the\npresence of Si(OH)4 groups, that permit the formation of a (Ca,P) layer.\nAluminium plays a role in the glass solubility and may inhibit apatite\nnucleation. After the beginning of the (Ca,P) layer formation, the size of the\nelectron dense (Si,O) layer decreases and tends to disappear. After two days of\nimmersion, the (Ca,P) layer grows in thickness and leads to apatite\nprecipitation",
"arxiv_id": "physics/0412146",
"authors": [
"V. Blanchet",
"E. Jallot",
"J. Michel",
"L. Wortham",
"D. Laurent-Maquin",
"G. Balossier"
],
"categories": [
"physics.bio-ph"
],
"journal_ref": "Surface and Interface Analysis 36 (2004) 658-665",
"title": "X-ray microanalysis in STEM of short-term physico-chemical reactions at bioactive glass particles / biological fluids interface. Determination of O/Si atomic ratios",
"url": "https://arxiv.org/abs/physics/0412146"
},
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