dorsal/arxiv
View SchemaGlial activation in white matter following ischemia in the neonatal P7 rat brain
| Authors | Valérie Biran, Luc-Marie Joly, Anne Héron, Agnès Vernet, Céline Véga, Jean Mariani, Sylvain Renolleau, Christiane Charriaut-Marlangue |
|---|---|
| Categories | |
| ArXiv ID | q-bio/0601011 |
| URL | https://arxiv.org/abs/q-bio/0601011 |
Abstract
This study examines cell death and proliferation in the white matter after neonatal stroke. In post-natal day 7 injured rat, there was a marked reduction in myelin basic protein (MBP) immunostaining mainly corresponding to numerous pyknotic immature oligodendrocytes and TUNEL-positive astrocytes in the ipsilateral external capsule. In contrast, a substantial restoration of MBP, as indicated by the MBP ratio of left-toright, occurred in the cingulum at 48 (1.27 +- 0.12) and 72 (1.30 +- 0.18, p<0.05) hours of recovery as compared to age-matched controls (1.03 +- 0.14). Ki-67 immunostaining revealed a first peak of newly-generated cells in the dorsolateral hippocampal subventricular zone and cingulum at 72 hours after reperfusion. Double immunofluorescence revealed that most of the Ki-67-positive cells were astrocytes at 48 hours and NG2 pre-oligodendrocytes at 72 hours of recovery. Microglia infiltration occurs over several days in the cingulum and a huge quantity of macrophages reached the subcortical white matter where they engulfed immature oligodendrocytes. The overall results suggest that the persistent activation of microglia involves a chronic component of immunoinflammation, which overwhelms repair processes and contributes to cystic growth in the developing brain.
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"abstract": "This study examines cell death and proliferation in the white matter after\nneonatal stroke. In post-natal day 7 injured rat, there was a marked reduction\nin myelin basic protein (MBP) immunostaining mainly corresponding to numerous\npyknotic immature oligodendrocytes and TUNEL-positive astrocytes in the\nipsilateral external capsule. In contrast, a substantial restoration of MBP, as\nindicated by the MBP ratio of left-toright, occurred in the cingulum at 48\n(1.27 +- 0.12) and 72 (1.30 +- 0.18, p\u003c0.05) hours of recovery as compared to\nage-matched controls (1.03 +- 0.14). Ki-67 immunostaining revealed a first peak\nof newly-generated cells in the dorsolateral hippocampal subventricular zone\nand cingulum at 72 hours after reperfusion. Double immunofluorescence revealed\nthat most of the Ki-67-positive cells were astrocytes at 48 hours and NG2\npre-oligodendrocytes at 72 hours of recovery. Microglia infiltration occurs\nover several days in the cingulum and a huge quantity of macrophages reached\nthe subcortical white matter where they engulfed immature oligodendrocytes. The\noverall results suggest that the persistent activation of microglia involves a\nchronic component of immunoinflammation, which overwhelms repair processes and\ncontributes to cystic growth in the developing brain.",
"arxiv_id": "q-bio/0601011",
"authors": [
"Val\u00e9rie Biran",
"Luc-Marie Joly",
"Anne H\u00e9ron",
"Agn\u00e8s Vernet",
"C\u00e9line V\u00e9ga",
"Jean Mariani",
"Sylvain Renolleau",
"Christiane Charriaut-Marlangue"
],
"categories": [
"q-bio.NC"
],
"title": "Glial activation in white matter following ischemia in the neonatal P7 rat brain",
"url": "https://arxiv.org/abs/q-bio/0601011"
},
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