dorsal/arxiv
View SchemaQualitative Assessment of Gene Expression in Affymetrix Genechip Arrays
| Authors | Radhakrishnan Nagarajan, Meenakshi Upreti |
|---|---|
| Categories | |
| ArXiv ID | q-bio/0604015 |
| URL | https://arxiv.org/abs/q-bio/0604015 |
| DOI | 10.1016/j.physa.2006.06.004 |
Abstract
Affymetrix Genechip microarrays are used widely to determine the simultaneous expression of genes in a given biological paradigm. Probes on the Genechip array are atomic entities which by definition are randomly distributed across the array and in turn govern the gene expression. In the present study, we make several interesting observations. We show that there is considerable correlation between the probe intensities across the array which defy the independence assumption. While the mechanism behind such correlations is unclear, we show that scaling behavior and the profiles of perfect match (PM) as well as mismatch (MM) probes are similar and immune to background subtraction. We believe that the observed correlations are possibly an outcome of inherent non-stationarities or patchiness in the array devoid of biological significance. This is demonstrated by inspecting their scaling behavior and profiles of the PM and MM probe intensities obtained from publicly available Genechip arrays from three eukaryotic genomes, namely: Drosophila Melanogaster, Homo Sapiens and Mus musculus across distinct biological paradigms and across laboratories, with and without background subtraction. The fluctuation functions were estimated using detrended fluctuation analysis (DFA) with fourth order polynomial detrending. The results presented in this study provide new insights into correlation signatures of PM and MM probe intensities and suggests the choice of DFA as a tool for qualitative assessment of Affymetrix Genechip microarrays prior to their analysis. A more detailed investigation is necessary in order to understand the source of these correlations.
{
"annotation_id": "28a08844-bb96-4bd1-a424-249ed8bf7787",
"date_created": "2026-03-02T18:01:35.302000Z",
"date_modified": "2026-03-02T18:01:35.302000Z",
"file_hash": "8de6f080986dfa14d912d64fb0d603775f3fabc9811f503a5866977445a1cc71",
"private": false,
"record": {
"abstract": "Affymetrix Genechip microarrays are used widely to determine the simultaneous\nexpression of genes in a given biological paradigm. Probes on the Genechip\narray are atomic entities which by definition are randomly distributed across\nthe array and in turn govern the gene expression. In the present study, we make\nseveral interesting observations. We show that there is considerable\ncorrelation between the probe intensities across the array which defy the\nindependence assumption. While the mechanism behind such correlations is\nunclear, we show that scaling behavior and the profiles of perfect match (PM)\nas well as mismatch (MM) probes are similar and immune to background\nsubtraction. We believe that the observed correlations are possibly an outcome\nof inherent non-stationarities or patchiness in the array devoid of biological\nsignificance. This is demonstrated by inspecting their scaling behavior and\nprofiles of the PM and MM probe intensities obtained from publicly available\nGenechip arrays from three eukaryotic genomes, namely: Drosophila Melanogaster,\nHomo Sapiens and Mus musculus across distinct biological paradigms and across\nlaboratories, with and without background subtraction. The fluctuation\nfunctions were estimated using detrended fluctuation analysis (DFA) with fourth\norder polynomial detrending. The results presented in this study provide new\ninsights into correlation signatures of PM and MM probe intensities and\nsuggests the choice of DFA as a tool for qualitative assessment of Affymetrix\nGenechip microarrays prior to their analysis. A more detailed investigation is\nnecessary in order to understand the source of these correlations.",
"arxiv_id": "q-bio/0604015",
"authors": [
"Radhakrishnan Nagarajan",
"Meenakshi Upreti"
],
"categories": [
"q-bio.GN"
],
"doi": "10.1016/j.physa.2006.06.004",
"title": "Qualitative Assessment of Gene Expression in Affymetrix Genechip Arrays",
"url": "https://arxiv.org/abs/q-bio/0604015"
},
"schema_id": "dorsal/arxiv",
"source": {
"execution_id": "86132d40-b9b6-402f-9c9b-fe92004d980b",
"id": "arXiv Dataset IDs",
"type": "Model",
"variant": "snapshot-2026-03-01",
"version": "0.1.0"
},
"user_id": 1000002
}