dorsal/arxiv
View SchemaA quantitative model for refilling of the sarcoplasmic reticulum during vascular smooth muscle asynchronous [Ca2+] oscillations
| Authors | Nicola Fameli, Cornelis Van Breemen, Kuo-Hsing Kuo |
|---|---|
| Categories | |
| ArXiv ID | q-bio/0603001 |
| URL | https://arxiv.org/abs/q-bio/0603001 |
| DOI | 10.1016/j.ceca.2007.02.001 |
| Journal | Cell Calcium 42, 565-75 (2007) |
Abstract
We have developed a quantitative model for the creation of cytoplasmic Ca2+ gradients near the inner surface of the plasma membrane (PM). In particular we simulated the refilling of the sarcoplasmic reticulum (SR) via PM-SR junctions during asynchronous [Ca2+] oscillations in smooth muscle cells of the rabbit inferior vena cava. We have combined confocal microscopy data on the [Ca2+] oscillations, force transduction data from cell contraction studies and electron microscopic images to build a basis for computational simulations that model the transport of calcium ions from Na+/Ca2+ exchangers (NCX) on the PM to sarcoplasmic/endoplasmic reticulum Ca2+ ATPase (SERCA) pumps on the SR as a three-dimensional random walk through the PM-SR junctional cytoplasmic spaces. Electron microscopic ultrastructural images of the smooth muscle cells were elaborated with software algorithms to produce a very clear and dimensionally accurate picture of the PM-SR junctions. From this study, we conclude that it is plausible and possible for enough Ca2+ to pass through the PM-SR junctions to replete the SR during the regenerative Ca2+ release, which underlies agonist induced asynchronous Ca2+ oscillations in vascular smooth muscle.
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"abstract": "We have developed a quantitative model for the creation of cytoplasmic Ca2+\ngradients near the inner surface of the plasma membrane (PM). In particular we\nsimulated the refilling of the sarcoplasmic reticulum (SR) via PM-SR junctions\nduring asynchronous [Ca2+] oscillations in smooth muscle cells of the rabbit\ninferior vena cava. We have combined confocal microscopy data on the [Ca2+]\noscillations, force transduction data from cell contraction studies and\nelectron microscopic images to build a basis for computational simulations that\nmodel the transport of calcium ions from Na+/Ca2+ exchangers (NCX) on the PM to\nsarcoplasmic/endoplasmic reticulum Ca2+ ATPase (SERCA) pumps on the SR as a\nthree-dimensional random walk through the PM-SR junctional cytoplasmic spaces.\nElectron microscopic ultrastructural images of the smooth muscle cells were\nelaborated with software algorithms to produce a very clear and dimensionally\naccurate picture of the PM-SR junctions. From this study, we conclude that it\nis plausible and possible for enough Ca2+ to pass through the PM-SR junctions\nto replete the SR during the regenerative Ca2+ release, which underlies agonist\ninduced asynchronous Ca2+ oscillations in vascular smooth muscle.",
"arxiv_id": "q-bio/0603001",
"authors": [
"Nicola Fameli",
"Cornelis Van Breemen",
"Kuo-Hsing Kuo"
],
"categories": [
"q-bio.QM"
],
"doi": "10.1016/j.ceca.2007.02.001",
"journal_ref": "Cell Calcium 42, 565-75 (2007)",
"title": "A quantitative model for refilling of the sarcoplasmic reticulum during vascular smooth muscle asynchronous [Ca2+] oscillations",
"url": "https://arxiv.org/abs/q-bio/0603001"
},
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