dorsal/arxiv
View SchemaA Kinetic Model Describing the Processivity of Myosin-V
| Authors | K. I. Skau, R. B. Hoyle, M. S Turner |
|---|---|
| Categories | |
| ArXiv ID | q-bio/0606014 |
| URL | https://arxiv.org/abs/q-bio/0606014 |
| DOI | 10.1529/biophysj.105.070888 |
Abstract
The precise details of how myosin-V coordinates the biochemical reactions and mechanical motions of its two head elements to engineer effective processive molecular motion along actin filaments remain unresolved. We compare a quantitative kinetic model of the myosin-V walk, consisting of five basic states augmented by two further states to allow for futile hydrolysis and detachments, with experimental results for run lengths, velocities, and dwell times and their dependence on bulk nucleotide concentrations and external loads in both directions. The model reveals how myosin-V can use the internal strain in the molecule to synchronise the motion of the head elements. Estimates for the rate constants in the reaction cycle and the internal strain energy are obtained by a computational comparison scheme involving an extensive exploration of the large parameter space. This scheme exploits the fact that we have obtained analytic results for our reaction network, e.g. for the velocity but also the run length, diffusion constant and fraction of backward steps. The agreement with experiment is often reasonable but some open problems are highlighted, in particular the inability of such a general model to reproduce the reported dependence of run length on ADP. The novel way that our approach explores parameter space means that any confirmed discrepancies should give new insights into the reaction network model.
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"abstract": "The precise details of how myosin-V coordinates the biochemical reactions and\nmechanical motions of its two head elements to engineer effective processive\nmolecular motion along actin filaments remain unresolved. We compare a\nquantitative kinetic model of the myosin-V walk, consisting of five basic\nstates augmented by two further states to allow for futile hydrolysis and\ndetachments, with experimental results for run lengths, velocities, and dwell\ntimes and their dependence on bulk nucleotide concentrations and external loads\nin both directions. The model reveals how myosin-V can use the internal strain\nin the molecule to synchronise the motion of the head elements. Estimates for\nthe rate constants in the reaction cycle and the internal strain energy are\nobtained by a computational comparison scheme involving an extensive\nexploration of the large parameter space. This scheme exploits the fact that we\nhave obtained analytic results for our reaction network, e.g. for the velocity\nbut also the run length, diffusion constant and fraction of backward steps. The\nagreement with experiment is often reasonable but some open problems are\nhighlighted, in particular the inability of such a general model to reproduce\nthe reported dependence of run length on ADP. The novel way that our approach\nexplores parameter space means that any confirmed discrepancies should give new\ninsights into the reaction network model.",
"arxiv_id": "q-bio/0606014",
"authors": [
"K. I. Skau",
"R. B. Hoyle",
"M. S Turner"
],
"categories": [
"q-bio.BM"
],
"doi": "10.1529/biophysj.105.070888",
"title": "A Kinetic Model Describing the Processivity of Myosin-V",
"url": "https://arxiv.org/abs/q-bio/0606014"
},
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