{
  "annotation_id": "0fd38f3e-d7c8-458e-9448-9799b562fe16",
  "date_created": "2026-03-02T18:01:28.588000Z",
  "date_modified": "2026-03-02T18:01:28.588000Z",
  "file_hash": "df330c737ce50811423c4584f825450a3597b8f3cbdf628ba9a21c6aa44b7db3",
  "private": false,
  "record": {
    "abstract": "Fluorescence Lifetime Imaging Microscopy (FLIM) using multiphoton excitation\ntechniques is now finding an important place in quantitative imaging of\nprotein-protein interactions and intracellular physiology. We review here the\nrecent developments in multiphoton FLIM methods and also present a description\nof a novel multiphoton FLIM system using a streak camera that was developed in\nour laboratory. We provide an example of a typical application of the system in\nwhich we measure the fluorescence resonance energy transfer between a\ndonor/acceptor pair of fluorescent proteins within a cellular specimen.",
    "arxiv_id": "q-bio/0309022",
    "authors": [
      "R. V. Krishnan",
      "A. Masuda",
      "V. E. Centoze",
      "B. Herman"
    ],
    "categories": [
      "q-bio.QM",
      "q-bio.CB"
    ],
    "doi": "10.1117/1.1577574",
    "journal_ref": "Journal of Biomedical Optics Vo.8, 362 (July 2003)",
    "title": "Quantitative Imaging of Protein-Protein Interactions by Multiphoton   Fluorescence Lifetime Imaging Microscopy using a Streak camera",
    "url": "https://arxiv.org/abs/q-bio/0309022"
  },
  "schema_id": "dorsal/arxiv",
  "source": {
    "execution_id": "5ba2aa06-0983-40fd-88cc-4c4bb08ed0ac",
    "id": "arXiv Dataset IDs",
    "type": "Model",
    "variant": "snapshot-2026-03-01",
    "version": "0.1.0"
  },
  "user_id": 1000002
}