dorsal/arxiv
View SchemaQuantitative Imaging of Protein-Protein Interactions by Multiphoton Fluorescence Lifetime Imaging Microscopy using a Streak camera
| Authors | R. V. Krishnan, A. Masuda, V. E. Centoze, B. Herman |
|---|---|
| Categories | |
| ArXiv ID | q-bio/0309022 |
| URL | https://arxiv.org/abs/q-bio/0309022 |
| DOI | 10.1117/1.1577574 |
| Journal | Journal of Biomedical Optics Vo.8, 362 (July 2003) |
Abstract
Fluorescence Lifetime Imaging Microscopy (FLIM) using multiphoton excitation techniques is now finding an important place in quantitative imaging of protein-protein interactions and intracellular physiology. We review here the recent developments in multiphoton FLIM methods and also present a description of a novel multiphoton FLIM system using a streak camera that was developed in our laboratory. We provide an example of a typical application of the system in which we measure the fluorescence resonance energy transfer between a donor/acceptor pair of fluorescent proteins within a cellular specimen.
{
"annotation_id": "0fd38f3e-d7c8-458e-9448-9799b562fe16",
"date_created": "2026-03-02T18:01:28.588000Z",
"date_modified": "2026-03-02T18:01:28.588000Z",
"file_hash": "df330c737ce50811423c4584f825450a3597b8f3cbdf628ba9a21c6aa44b7db3",
"private": false,
"record": {
"abstract": "Fluorescence Lifetime Imaging Microscopy (FLIM) using multiphoton excitation\ntechniques is now finding an important place in quantitative imaging of\nprotein-protein interactions and intracellular physiology. We review here the\nrecent developments in multiphoton FLIM methods and also present a description\nof a novel multiphoton FLIM system using a streak camera that was developed in\nour laboratory. We provide an example of a typical application of the system in\nwhich we measure the fluorescence resonance energy transfer between a\ndonor/acceptor pair of fluorescent proteins within a cellular specimen.",
"arxiv_id": "q-bio/0309022",
"authors": [
"R. V. Krishnan",
"A. Masuda",
"V. E. Centoze",
"B. Herman"
],
"categories": [
"q-bio.QM",
"q-bio.CB"
],
"doi": "10.1117/1.1577574",
"journal_ref": "Journal of Biomedical Optics Vo.8, 362 (July 2003)",
"title": "Quantitative Imaging of Protein-Protein Interactions by Multiphoton Fluorescence Lifetime Imaging Microscopy using a Streak camera",
"url": "https://arxiv.org/abs/q-bio/0309022"
},
"schema_id": "dorsal/arxiv",
"source": {
"execution_id": "5ba2aa06-0983-40fd-88cc-4c4bb08ed0ac",
"id": "arXiv Dataset IDs",
"type": "Model",
"variant": "snapshot-2026-03-01",
"version": "0.1.0"
},
"user_id": 1000002
}