dorsal/arxiv
View SchemaAdsorption models of hybridization and post-hybridisation behaviour on oligonucleotide microarrays
| Authors | C. J. Burden, Y. Pittelkow, S. R. Wilson |
|---|---|
| Categories | |
| ArXiv ID | q-bio/0411005 |
| URL | https://arxiv.org/abs/q-bio/0411005 |
| DOI | 10.1088/0953-8984/18/23/024 |
| Journal | J. Phys.: Condens. Matter 18 (2006) 5545-5565 |
Abstract
Analysis of data from an Affymetrix Latin Square spike-in experiment indicates that measured fluorescence intensities of features on an oligonucleotide microarray are related to spike-in RNA target concentrations via a hyperbolic response function, generally identified as a Langmuir adsorption isotherm. Furthermore the asymptotic signal at high spike-in concentrations is almost invariably lower for a mismatch feature than for its partner perfect match feature. We survey a number of theoretical adsorption models of hybridization at the microarray surface and find that in general they are unable to explain the differing saturation responses of perfect and mismatch features. On the other hand, we find that a simple and consistent explanation can be found in a model in which equilibrium hybridization followed by partial dissociation of duplexes during the post-hybridization washing phase.
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"abstract": "Analysis of data from an Affymetrix Latin Square spike-in experiment\nindicates that measured fluorescence intensities of features on an\noligonucleotide microarray are related to spike-in RNA target concentrations\nvia a hyperbolic response function, generally identified as a Langmuir\nadsorption isotherm. Furthermore the asymptotic signal at high spike-in\nconcentrations is almost invariably lower for a mismatch feature than for its\npartner perfect match feature. We survey a number of theoretical adsorption\nmodels of hybridization at the microarray surface and find that in general they\nare unable to explain the differing saturation responses of perfect and\nmismatch features. On the other hand, we find that a simple and consistent\nexplanation can be found in a model in which equilibrium hybridization followed\nby partial dissociation of duplexes during the post-hybridization washing\nphase.",
"arxiv_id": "q-bio/0411005",
"authors": [
"C. J. Burden",
"Y. Pittelkow",
"S. R. Wilson"
],
"categories": [
"q-bio.BM"
],
"doi": "10.1088/0953-8984/18/23/024",
"journal_ref": "J. Phys.: Condens. Matter 18 (2006) 5545-5565",
"title": "Adsorption models of hybridization and post-hybridisation behaviour on oligonucleotide microarrays",
"url": "https://arxiv.org/abs/q-bio/0411005"
},
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